Document Detail


Improved fertility in gilts and sows after artificial insemination of frozen-thawed boar semen by supplementation of semen extender with caffeine and CaCl2.
MedLine Citation:
PMID:  19734696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Supplementation of semen extender with caffeine and CaCl(2) for artificial insemination (AI) of fresh spermatozoa has been demonstrated to reduce recruitment of uterine polymorphonuclear leukocytes (PMNs) and the activity of phagocytosis. Here, we determined if addition of caffeine and CaCl(2) to semen extender improves the fertility of frozen-thawed boar semen. In experiment 1, gilts were cervically inseminated twice with frozen-thawed boar spermatozoa (25 x 10(8) cells per dose) suspended in Modena solution (n=7) or modified Beltsville Thawing Solution supplemented with caffeine and CaCl(2) (BCC, n=7). The gilts were slaughtered 4 h later, and their oviducts and uterine horns plus the body of the uterus were flushed to recover PMNs and non-phagocytosed spermatozoa. There was no difference in the total number of uterine PMNs between gilts inseminated with Modena solution and those inseminated with BCC (3.8 x 10(8) vs. 1.5 x 10(8) cells, respectively); however, the total number of uterine spermatozoa was higher when gilts were inseminated with BCC (40.6 x 10(6) cells) compared with those inseminated with Modena solution (1.4 x 10(6) cells, P<0.05). In experiment 2, gilts and sows were subjected to intrauterine insemination twice with frozen-thawed spermatozoa suspended (25 x 10(8) sperm per dose) in Modena (n=21) or BCC (n=21). The overall pregnancy and farrowing rates were higher in females inseminated with BCC (71.4 and 61.9%, respectively) compared with those inseminated with Modena solution (38.1 and 28.6%, respectively, P<0.05). However, no significant difference in litter size of piglets was observed between treatments (7.2 +/- 1.6 piglets for Modena solution vs. 8.2 +/- 0.9 piglets for BCC solution). In conclusion, we demonstrated that use of BCC solution for frozen-thawed boar semen produced better pregnancy and farrowing rates following AI than Modena solution, probably by reducing the phagocytosis of spermatozoa.
Authors:
Shoichiro Yamaguchi; Hiroaki Funahashi; Tetsuya Murakami
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2009-09-07
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  55     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-15     Completed Date:  2010-03-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  645-9     Citation Subset:  IM    
Affiliation:
Fukuoka Agricultural Research Center, Fukuoka, Japan. yamashou@farc.pref.fukuoka.jp
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MeSH Terms
Descriptor/Qualifier:
Animal Husbandry / methods,  statistics & numerical data
Animals
Caffeine / pharmacology*
Calcium Chloride / pharmacology*
Cell Survival / drug effects
Cryopreservation / methods,  veterinary*
Cryoprotective Agents / pharmacology*
Drug Synergism
Female
Fertilization / drug effects
Insemination, Artificial / methods,  veterinary*
Live Birth
Male
Oviducts / cytology,  drug effects
Pregnancy
Pregnancy Rate
Semen Preservation / methods,  veterinary*
Sperm Transport / drug effects
Sus scrofa / physiology*
Uterus / cytology,  drug effects,  immunology
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 10043-52-4/Calcium Chloride; 58-08-2/Caffeine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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