Document Detail


Improved Detection of Polygalacturonase Activity due to Mucor piriformis with a Modified Dinitrosalicylic Acid Reagent.
MedLine Citation:
PMID:  18945136     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT An assay for determination of galacturonic acid with 3,5-dinitrosalicylic acid was developed that substantially extends the linear range of detection compared to a previously published method with this reagent. In the improved assay, galacturonic acid was detected with a reagent containing 44 mM 3,5-dinitrosalicylic acid, 4 mM sodium sulfite, and 375 mM sodium hydroxide. The absorbance of the solution after reaction with galacturonic acid was determined at 575 nm and was linear at concentrations of galacturonic acid up to 50 mumol, with a lower limit of detection at ~400 nmol. The assay with the improved reagent could be performed in wavelength ranges from 550 to 575 nm, with higher sensitivity at the shorter wavelengths. The new reagent was used in routine assays of polygalacturonase activity in culture filtrates of the important postharvest fungal pathogen Mucor piriformis.
Authors:
G Wang; T J Michailides; R M Bostock
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytopathology     Volume:  87     ISSN:  0031-949X     ISO Abbreviation:  Phytopathology     Publication Date:  1997 Feb 
Date Detail:
Created Date:  2008-10-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9427222     Medline TA:  Phytopathology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  161-3     Citation Subset:  -    
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