Document Detail


Improved DNA vaccination by skin-targeted delivery using dry-coated densely-packed microprojection arrays.
MedLine Citation:
PMID:  20850487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HSV-2-gD2 DNA vaccine was precisely delivered to immunologically sensitive regions of the skin epithelia using dry-coated microprojection arrays. These arrays delivered a vaccine payload to the epidermis and the upper dermis of mouse skin. Immunomicroscopy results showed that, in 43 ± 5% of microprojection delivery sites, the DNA vaccine was delivered to contact with professional antigen presenting cells in the epidermal layer. Associated with this efficient delivery of the vaccine into the vicinity of the professional antigen presenting cells, we achieved superior antibody responses and statistically equal protection rate against an HSV-2 virus challenge, when compared with the mice immunized with intramuscular injection using needle and syringe, but with less than 1/10th of the delivered antigen.
Authors:
Xianfeng Chen; Angela Shaulov Kask; Michael L Crichton; Celia McNeilly; Sally Yukiko; Lichun Dong; Joshua O Marshak; Courtney Jarrahian; Germain J P Fernando; Dexiang Chen; David M Koelle; Mark A F Kendall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-17
Journal Detail:
Title:  Journal of controlled release : official journal of the Controlled Release Society     Volume:  148     ISSN:  1873-4995     ISO Abbreviation:  J Control Release     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-06     Completed Date:  2011-03-25     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  8607908     Medline TA:  J Control Release     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  327-33     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Administration, Cutaneous
Animals
Antibody Formation
Drug Delivery Systems / instrumentation*
Female
Herpes Genitalis / prevention & control*
Herpesvirus 2, Human / immunology*
Mice
Mice, Inbred BALB C
Skin / metabolism*
Skin Absorption
Vaccination / instrumentation*
Vaccines, DNA / administration & dosage*
Chemical
Reg. No./Substance:
0/Vaccines, DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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