Document Detail

Improved 4- and 6-hour myocardial preservation by hypoxic preconditioning.
MedLine Citation:
PMID:  7586448     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: A brief hypoxic episode can precondition myocardium against a subsequent ischemic-reperfusion injury. The present study sought to determine whether intracellular ionic alterations, induced expression of heat-shock proteins (hsps), and/or catalase are involved in the cellular mechanisms by which hypoxic preconditioning can preserve postischemic function in a model of prolonged hypothermic storage. METHODS AND RESULTS: Two groups of isolated working rat hearts were studied: control (CON) and hypoxically preconditioned (HP) hearts. Hearts were arrested at 4 degrees C with St Thomas' cardioplegic solution and immersion-stored for either a 4- or 6-hour period. Myocardial function (ie, heart rate, aortic flow, coronary flow, developed pressure, and its first derivative dP/dtmax) was determined at baseline, after preconditioning, and during reperfusion. At similar time points, myocardial [Na+]i, [K+]i, [Mg2+]i, and [Ca2+]i were measured using an atomic absorption spectrophotometer, and the induction of hsp 70 and catalase mRNAs was assayed using Northern blot analysis. After 4 and 6 hours of hypothermic storage, aortic flow, dP/dtmax, and [K+]i were increased, whereas [Na+]i and [Ca2+]i were decreased significantly in the HP group compared with the CON group. Steady state mRNA levels of catalase and hsp 70 were increased from baseline levels only in the HP group, with a peak (2.8- and 2.4-fold versus baseline) after 4 hours of storage. CONCLUSIONS: Our results indicate that intracellular ionic alterations and upregulation of catalase and hsp 70 gene expression may contribute to the mechanisms underlying hypoxic preconditioning, leading to improved postischemic function during prolonged hypothermic storage of hearts.
D T Engelman; C Z Chen; M Watanabe; R M Engelman; J A Rousou; J E Flack; D W Deaton; N Maulik; D K Das
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation     Volume:  92     ISSN:  0009-7322     ISO Abbreviation:  Circulation     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-28     Completed Date:  1995-12-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  II417-22     Citation Subset:  AIM; IM    
Department of Surgery, University of Connecticut School of Medicine, Farmington, USA.
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MeSH Terms
Anoxia / physiopathology*
Catalase / metabolism
Electrolytes / metabolism
HSP70 Heat-Shock Proteins / metabolism
Heart* / physiopathology
Myocardium / metabolism
Organ Preservation*
Rats, Sprague-Dawley
Time Factors
Grant Support
Reg. No./Substance:
0/Electrolytes; 0/HSP70 Heat-Shock Proteins; EC

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