Document Detail

Impression cytology following mitomycin C therapy for ocular surface squamous neoplasia.
MedLine Citation:
PMID:  11520767     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Topical mitomycin C (MMC) therapy has been used for treatment of ocular surface squamous neoplasia (OSSN) since 1994. Relatively few studies have reported the cellular changes in ocular surface following MMC. METHODS: Impression cytology was studied in four patients with ocular surface squamous neoplasia, either primary or recurrence after previous excisional biopsy. The authors studied samples obtained using Millipore filters at intervals between 4 and 17 weeks after commencement of MMC, and compared them with pretreatment cytology. RESULTS: MMC induced changes of cytomegaly, cytoplasmic vacuolation, nucleomegaly with nuclear wrinkling, and binucleation or multinucleation were seen in some cells in all samples. However, nuclear/cytoplasmic (N/C) ratio in these enlarged cells was normal. These changes mimicked those seen following radiation therapy in uterine cervix. Changes of increased nuclear and cell size with increased N/C ratio were seen in some dysplastic cells. The predominant form of cell death was apoptosis with fewer cells showing necrosis. CONCLUSIONS: MMC appears to produce cell death in OSSN by apoptosis and necrosis. Cellular changes related to MMC mimic those caused by radiation-cytomegaly, nucleomegaly, and vacuolation. MMC related changes may persist in ocular surface epithelium for at least 8 months following MMC therapy.
P A McKelvie; M Daniell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The British journal of ophthalmology     Volume:  85     ISSN:  0007-1161     ISO Abbreviation:  Br J Ophthalmol     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-08-24     Completed Date:  2001-09-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0421041     Medline TA:  Br J Ophthalmol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1115-9     Citation Subset:  IM    
Department of Anatomical Pathology, St Vincent's Hospital, Melbourne, Victoria, Australia.
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MeSH Terms
Antibiotics, Antineoplastic / pharmacology*,  therapeutic use
Apoptosis / drug effects
Carcinoma, Squamous Cell / drug therapy*,  pathology
Conjunctival Neoplasms / drug therapy,  pathology
Corneal Diseases / drug therapy,  pathology
Eye / drug effects*,  pathology
Eye Neoplasms / drug therapy*,  pathology
Mitomycin / pharmacology*,  therapeutic use
Nucleic Acid Synthesis Inhibitors / pharmacology,  therapeutic use
Prospective Studies
Retrospective Studies
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Nucleic Acid Synthesis Inhibitors; 50-07-7/Mitomycin

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