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Importance of subaerial biofilms and airborne microflora in the deterioration of stonework: a molecular study.
MedLine Citation:
PMID:  23025579     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The study characterized the sessile microbial communities on mortar and stone in Milan University's Richini's Courtyard and investigated the relationship between airborne and surface-associated microbial communities. Active colonization was found in three locations: green and black patinas were present on mortar and black spots on stone. Confocal laser scanning microscopy, scanning electron microscopy and culture-independent molecular methods revealed that the biofilm causing deterioration was dominated by green algae and black fungi. The mortar used for restoration contained acrylic and siloxane resins that could be used by microorganisms as carbon and energy sources thereby causing proliferation of the biofilm. Epifluorescence microscopy and culture-based methods highlighted a variety of airborne microflora. Bacterial and fungal counts were quantitatively similar to those reported in other investigations of urban areas, the exception being fungi during summer (1-2 orders of magnitude higher). For the first time in the cultural heritage field, culture-independent molecular methods were used to resolve the structure of airborne communities near discoloured surfaces, and to investigate the relationship between such communities and surface-associated biofilms.
Authors:
Andrea Polo; Davide Gulotta; Nadia Santo; Cristiano Di Benedetto; Umberto Fascio; Lucia Toniolo; Federica Villa; Francesca Cappitelli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biofouling     Volume:  28     ISSN:  1029-2454     ISO Abbreviation:  Biofouling     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200331     Medline TA:  Biofouling     Country:  England    
Other Details:
Languages:  eng     Pagination:  1093-106     Citation Subset:  IM    
Affiliation:
a Dipartimento di Scienze per gli Alimenti , la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano , Via Celoria 2 , 20133 Milano , Italy.
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