| Importance of storage conditions for the stability of zinc- and cadmium-induced metallothionein. | |
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MedLine Citation:
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PMID: 11575683 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the storage stability of metallothionein (MT), a cysteine-rich protein that has diagnostic potential as a cancer marker and in the assessment of Zn status and heavy-metal toxicity. MT was rapidly degraded in samples of rat whole liver at -20 degrees C or -70 degrees C. MT in supernatants from heat-treated rat liver homogenates stored as 1:5 dilutions of liver from Zn- or Cd-induced rats were stable (recovery >98%) for 100 d at temperatures of -70 degrees C and -196 degrees C but not at -20 degrees C, regardless of the presence of dithiothreitol (DTT) or argon. The variability of MT measurement by the 109Cd-hemoglobin affinity assay was however greatest in samples from Zn-induced rats stored without DTT. The integrity of the MT protein in supernatants of heat-treated homogenates stored for 100 d was demonstrated by Sephadex G-75 chromatography. When heat-treated supernatants were stored as dilute solutions (1:125 of liver), MT was unstable regardless of treatment or storage temperature. Our findings show that liver MT is stable for at least 4 mo as a supernatant of a heat-treated homogenate (1:5 dilution of liver) when stored at or below -70 degrees C and in the presence of DTT. |
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Authors:
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P Coyle; C A Hubert; J C Philcox; A M Rofe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biological trace element research Volume: 81 ISSN: 0163-4984 ISO Abbreviation: Biol Trace Elem Res Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-09-28 Completed Date: 2002-03-04 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7911509 Medline TA: Biol Trace Elem Res Country: United States |
Other Details:
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Languages: eng Pagination: 269-78 Citation Subset: IM |
Affiliation:
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Division of Clinical Biochemistry, Institute of Medical and Veterinary Science, Adelaide, SA, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Argon / pharmacology Cadmium / chemistry* Chromatography Dextrans / chemistry Dithiothreitol / pharmacology Drug Stability Drug Storage Hemoglobins / metabolism Hot Temperature Liver / metabolism Male Metallothionein / chemistry* Rats Temperature Time Factors Zinc / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins; 3483-12-3/Dithiothreitol; 7440-37-1/Argon; 7440-43-9/Cadmium; 7440-66-6/Zinc; 9004-54-0/Dextrans; 9014-76-0/sephadex; 9038-94-2/Metallothionein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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