Document Detail


Importance of nitric oxide in canine femoral circulation: comparison of two NO inhibitors.
MedLine Citation:
PMID:  1638567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The aim was to assess the importance of endothelium derived nitric oxide (NO) in the regulation of vascular tone in the limbs. Changes in the canine femoral circulation were investigated after inhibition of NO synthesis. METHODS: The effects of two NO inhibitors, NG-monomethyl-L-arginine (LNMMA) and NG-nitro-L-arginine (NOARG), were compared on basal femoral blood flow and on endothelium dependent (acetylcholine) and endothelium independent (glyceryl trinitrate) vasodilatation in 15 pentobarbitone anaesthetised mongrel dogs. An electromagnetic flow probe was placed on the femoral artery to measure femoral blood flow. One catheter was advanced into the femoral artery proximal to the flow probe for blood pressure recording and another catheter distal to the flow probe for drug infusions. RESULTS: LNMMA (0.28 mumol.ml-1) reduced basal femoral blood flow by 44(SEM 3)%, NOARG (0.07 mumol.ml-1) by 21(4)%, and NOARG (0.56 mumol.ml-1) by 29(3)%. The flow responses to acetylcholine were reduced after LNMMA by 27(8)%, unaltered after NOARG (0.07 mumol.ml-1), and reduced after NOARG (0.56 mumol.ml-1) by 60(7)%. The flow response to glyceryl trinitrate was unaltered. L-arginine re-established femoral blood flow after infusion of LNMMA and NOARG (0.07 mumol.ml-1), but L-arginine did not re-establish femoral blood flow after NOARG (0.56 mumol.ml-1), even when infused in a 60-fold molar excess. CONCLUSIONS: There is a continuous basal release of NO in the canine femoral circulation. The results obtained by infusing LNMMA suggest that more than 40% of basal femoral blood flow is mediated by endothelium derived NO. Whereas LNMMA was more potent than NOARG in reducing basal NO release, NOARG (0.56 mumol.ml-1) reduced acetylcholine induced vasodilatation by as much as 60%.
Authors:
K A Kirkebøen; P A Naess; G Christensen; F Kiil
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cardiovascular research     Volume:  26     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-08-28     Completed Date:  1992-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  357-61     Citation Subset:  IM    
Affiliation:
University of Oslo, Institute for Experimental Medical Research, Ullevål Hospital, Norway.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology
Animals
Arginine / analogs & derivatives*,  pharmacology
Dogs
Female
Femoral Artery / physiology*
Male
Nitric Oxide / metabolism*
Nitroarginine
Nitroglycerin / pharmacology
Regional Blood Flow / drug effects
Vasodilation / drug effects*
omega-N-Methylarginine
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; 17035-90-4/omega-N-Methylarginine; 2149-70-4/Nitroarginine; 51-84-3/Acetylcholine; 55-63-0/Nitroglycerin; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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