| Importance of the biopsy date in autologous endometrial cocultures for patients with multiple implantation failures. | |
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MedLine Citation:
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PMID: 12057730 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To analyze the effectiveness of autologous endometrial coculture by the cycle day of the endometrial biopsy. DESIGN: Retrospective study. SETTING: University-based IVF center. PATIENT(S): Two hundred eight patients with multiple IVF failures. INTERVENTION(S): Embryos were split and randomly allocated to growth on autologous endometrial coculture or conventional media. MAIN OUTCOME MEASURE(S): Embryo quality and pregnancy outcome. RESULT(S): The overall clinical pregnancy rate was 41.8%. Embryos grown on autologous endometrial coculture were of higher quality (more blastomeres and less fragmentation) than embryos grown with conventional media. Early luteal biopsies (<5 days after LH surge) for autologous endometrial coculture did not demonstrate an improvement in embryo quality as compared to the significant improvement demonstrated with later luteal endometrial biopsies (> or =5 days after LH surge). The date of the biopsy was predictive of pregnancy outcome when using autologous endometrial coculture (44.7% [> or =5 days after LH surge] vs. 18.8% [<5 days after LH surge], P=.012). CONCLUSION(S): We have demonstrated an improvement in embryo quality when using autologous endometrial coculture. The improvement in embryo quality and higher pregnancy rates were limited to biopsies > or =5 days after the LH surge. This suggests that mid/late luteal phase endometrium contains factors that enhanced embryo growth and subsequent implantation. |
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Authors:
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Steven D Spandorfer; Larry I Barmat; Jose Navarro; Hung-Ching Liu; Lucinda Veeck; Zev Rosenwaks |
Publication Detail:
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Type: Clinical Trial; Journal Article; Randomized Controlled Trial |
Journal Detail:
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Title: Fertility and sterility Volume: 77 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-06-11 Completed Date: 2002-07-03 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 1209-13 Citation Subset: IM |
Affiliation:
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The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Presbyterian Hospital, Weill Cornell Medical Center, New York, New York 10021, USA. sdspando@mail.med.cornell.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Biopsy / methods Blastocyst / physiology Coculture Techniques Culture Techniques Embryo Implantation* Endometrium / pathology*, physiopathology* Female Fertilization in Vitro* Humans Infertility / pathology*, physiopathology*, therapy Luteinizing Hormone / secretion Menstrual Cycle / physiology* Predictive Value of Tests Pregnancy Pregnancy Outcome Pregnancy Rate Retrospective Studies Time Factors Treatment Failure |
| Chemical | |
Reg. No./Substance:
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9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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