Document Detail


Importance of 3-hydroxy fatty acid composition of lipopeptides for biosurfactant activity.
MedLine Citation:
PMID:  16332741     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biosurfactant production may be an economic approach to improving oil recovery. To obtain candidates most suitable for oil recovery, 207 strains, mostly belonging to the genus Bacillus, were tested for growth and biosurfactant production in medium with 5% NaCl under aerobic and anaerobic conditions. All strains grew aerobically with 5% NaCl, and 147 strains produced a biosurfactant. Thirty-five strains grew anaerobically with 5% NaCl, and two produced a biosurfactant. In order to relate structural differences to activity, eight lipopeptide biosurfactants with different specific activities produced by various Bacillus species were purified by a new protocol. The amino acid compositions of the eight lipopeptides were the same (Glu/Gln:Asp/Asn:Val:Leu, 1:1:1:4), but the fatty acid compositions differed. Multiple regression analysis showed that the specific biosurfactant activity depended on the ratios of both iso to normal even-numbered fatty acids and anteiso to iso odd-numbered fatty acids. A multiple regression model accurately predicted the specific biosurfactant activities of four newly purified biosurfactants (r2= 0.91). The fatty acid composition of the biosurfactant produced by Bacillus subtilis subsp. subtilis strain T89-42 was altered by the addition of branched-chain amino acids to the growth medium. The specific activities of biosurfactants produced in cultures with different amino acid additions were accurately predicted by the multiple regression model derived from the fatty acid compositions (r2= 0.95). Our work shows that many strains of Bacillus mojavensis and Bacillus subtilis produce biosurfactants and that the fatty acid composition is important for biosurfactant activity.
Authors:
Noha H Youssef; Kathleen E Duncan; Michael J McInerney
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  71     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-07     Completed Date:  2006-02-07     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7690-5     Citation Subset:  IM    
Affiliation:
Department of Botany and Microbiology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Bacillus / classification*,  growth & development
Fatty Acids / analysis
Hydroxy Acids / analysis*
Lipoproteins / metabolism*
Surface-Active Agents*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fatty Acids; 0/Hydroxy Acids; 0/Lipoproteins; 0/Surface-Active Agents
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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