Document Detail


Import of lyso-phosphatidylcholine into chloroplasts likely at the origin of eukaryotic plastidial lipids.
MedLine Citation:
PMID:  10712548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plastids rely on the import of extraplastidial precursor for the synthesis of their own lipids. This key phenomenon in the formation of plastidial phosphatidylcholine (PC) and of the most abundant lipids on earth, namely galactolipids, is poorly understood. Various suggestions have been made on the nature of the precursor molecule(s) transferred to plastids, but despite general agreement that PC or a close metabolite plays a central role, there is no clear-cut answer to this question because of a lack of conclusive experimental data. We therefore designed experiments to discriminate between a transfer of PC, 1-acylglycero phosphorylcholine (lyso-PC), or glycerophosphorylcholine. After pulse-chase experiments with glycerol and acetate, plastids of leek (Allium porrum L.) seedlings were purified. The labels of the glycerol moiety and the sn-1- and sn-2-bound fatty acids of plastidial lipids were determined and compared with those associated with the extraplastidial PC. After import, plastid lipids contained the glycerol moiety and the fatty acids esterified to the sn-1 position originating from the extraplastidial PC; no import of sn-2-bound fatty acid was detected. These results rule out a transfer of PC or glycerophosphorylcholine, and are totally explained by an import of lyso-PC molecules used subsequently as precursor for the synthesis of eukaryotic plastid lipids.
Authors:
S Mongrand; C Cassagne; J J Bessoule
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  122     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-05-25     Completed Date:  2000-05-25     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  845-52     Citation Subset:  IM    
Affiliation:
Unité Mixte de Recherche 5544, Centre National de la Recherche Scientifique, Université Victor Segalen Bordeaux 2, 146, rue Léo Saignat-Case 92, 33076 Bordeaux cédex, France.
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MeSH Terms
Descriptor/Qualifier:
Allium / metabolism
Biological Transport, Active
Chloroplasts / metabolism*
Eukaryotic Cells
Fatty Acids / metabolism
Galactolipids
Glycolipids / metabolism
Kinetics
Lipid Metabolism
Lysophosphatidylcholines / metabolism*
Plastids / metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Galactolipids; 0/Glycolipids; 0/Lysophosphatidylcholines
Comments/Corrections

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