Document Detail


Implications for the use of acid preservatives in 24-hour urine for measurements of high demand biochemical analytes in clinical laboratories.
MedLine Citation:
PMID:  21910978     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Evaluate the level of interference of biochemists dosages in the 24-hour urine using or not the 6mol/l HCl acid in different concentrations and conditions and its implications in the most demanded analytes in clinical laboratory. METHODS: Twenty-two volunteers collected three 24-hour urine in 3 conditions: with 5ml/l and 20ml/l of 6mol/l HCl in the container, and without acid preservative. The samples collected without preservative were separated in aliquots and added 5ml/l of 6mol/l HCl after 24h. Analytes, uric acid creatinine, urea, chlorides, glucose, magnesium, sodium, potassium, microalbumin, proteins, amylase, aldosterone, calcium, cortisol, phosphorus, citric acid, oxalate, and metanephrines, were determined. RESULTS: Uric acid, glucose, microalbumin, protein, amylase and aldosterone showed that %CV ranging from 16 to 57% in the presence of acid preservative. Analytes that need acid preservative cortisol, citric acid and oxalate showed %CV ranging from 6 to 27% with r=0.66, r=0.77, r=0.70 respectively provided 5ml/l after delivery and r=0.31, r=0.70 and r=0.48 without preservative acid when compared with the gold standard (with 20ml/l of 6mol/l HCl). CONCLUSIONS: Glucose, microalbumin, protein, amylase and aldosterone urinary did not show good performance in the presence of acid preservative. Analytes that need acid preservative showed variation acceptable in condition 5ml/l of 6mol/l HCl added after 24h.
Authors:
Marcia C Feres; Rolando Bini; Maria Cristina De Martino; Simone P Biagini; Altay Lino de Sousa; Paulo G Campana; Sergio Tufik
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-2
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  -     ISSN:  1873-3492     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Affiliation:
Psychobiology Department of the Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
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