Document Detail

Implications on glycobiological aspects of tumor hypoxia in breast ductal carcinoma in situ.
MedLine Citation:
PMID:  23381603     Owner:  NLM     Status:  Publisher    
Breast carcinoma is one of the most common neoplasia and the first cause of women cancer related deaths worldwide. In the past few years with diagnostic increment, the number of patients diagnosed with ductal carcinoma in situ (DCIS) increased considerably and opened up new ways in research and new dilemmas in diagnostic and clinical practice. This work aimed to evaluate differences in Galectin-1 and Galectin-3 expression and lectins ligands profile on DCIS cells in hypoxic microenvironment. Lectin histochemistry and immunohistochemistry were performed with Concanavalin A, Wheat Germ Agglutinin, Peanut Agglutinin and Ulex europaeus Agglutinin lectins and with anti-Galectin-1 and anti-Galectin-3 antibodies. Lectin ligands were more recognized in hypoxic lesions by Concanavalin A (p = 0.0019), Wheat Germ Agglutinin (p < 0.001) and Ulex europaeus Agglutinin (p = 0.0014), but not by Peanut Agglutinin (p = 0.5779) when compared to non-hypoxic. Galectin-1 was not observed in all cases analyzed on both groups, differing from Galectin-3 that was overexpressed on cytoplasm of DCIS hypoxic group in relation to control group (p = 0.031). As far as we are concerned, this is the first paper that describes glycobiological alterations in breast cancer hypoxic environment in vivo that could be used to validate in vitro models on this aspect. Moreover, comedogenic/necrotic carcinomas were usually associated with poor-prognostic than others, and our results show that glycosylation may play an important role in this event.
Moacyr Jesus Barreto de Melo Rêgo; Gabriela Souto Vieira de Mello; Carlos André da Silva Santos; Roger Chammas; Eduardo Isidoro Carneiro Beltrão
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-5
Journal Detail:
Title:  Medical molecular morphology     Volume:  -     ISSN:  1860-1499     ISO Abbreviation:  Med Mol Morphol     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101239023     Medline TA:  Med Mol Morphol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Pathology Sector of Keizo Asami Immunopathology Laboratory-Federal University of Pernambuco (LIKA-UFPE), Recife, Brazil,
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