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Implication of the mineralocorticoid axis in rainbow trout osmoregulation during salinity acclimation.
MedLine Citation:
PMID:  21343325     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cortisol and the glucocorticoid receptors (GRs) play an important role in fish osmoregulation, while the involvement of the mineralocorticoid receptor (MR) and its putative ligand 11-deoxycorticosterone (DOC) has been poorly investigated. In this study, we assessed the implication of DOC and MR in rainbow trout (Oncorhynchus mykiss) osmoregulation during hypo- and hypersaline acclimation in parallel with the cortisol-GR system. A DOC RIA was developed to measure plasma DOC levels and a MR specific antibody was developed to localize MR protein in gill, intestine and kidney. This is the first study to report DOC plasma levels during salinity change and MR localization in fish osmoregulatory tissue. Corticosteroid receptor mRNA abundance was investigated in osmoregulatory tissue during salinity acclimation and the effect of cortisol and DOC on ionic transporters gene expression was assayed using an in vitro gill incubation method. Differential tissue-, salinity- and time-dependent changes in MR mRNA levels during both hyper- and hyposaline acclimation and the ubiquitous localization of MR in osmoregulatory tissue suggest a role for the MR in osmoregulation. Presumably, DOC does not act as ligand for MR in osmoregulation since there were no changes in plasma DOC levels during either FW-SW or SW-FW acclimation or any effect of DOC on gill ionic transporter mRNA levels in the gill. Taken together, these results suggest a role for MR, but not DOC, in osmoregulation and confirm the importance of cortisol as a major endocrine regulator of trout osmoregulation.
Authors:
Patrick Prunet; Pia Kiilerich; Sylvain Milla; Armin Sturm; Xavier Terrien; Sylvie Chevolleau; Franck Giton; Jean Fiet; Claudiane Valotaire; Alexis Fostier; Laurent Debrauwer
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-22
Journal Detail:
Title:  The Journal of endocrinology     Volume:  -     ISSN:  1479-6805     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
P Prunet, INRA-SCRIBE, Rennes, France.
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