Document Detail


Implication of the glutamine synthetase/glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves.
MedLine Citation:
PMID:  18479460     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the role of glutamine synthetases (cytosolic GS1 and chloroplast GS2) and glutamate synthases (ferredoxin-GOGAT and NADH-GOGAT) in the inorganic nitrogen assimilation and reassimilation into amino acids between bundle sheath cells and mesophyll cells for the remobilization of amino acids during the early phase of grain filling in Zea mays L. The plants responded to a light/dark cycle at the level of nitrate, ammonium and amino acids in the second leaf, upward from the primary ear, which acted as the source organ. The assimilation of ammonium issued from distinct pathways and amino acid synthesis were evaluated from the diurnal rhythms of the transcripts and the encoded enzyme activities of nitrate reductase, nitrite reductase, GS1, GS2, ferredoxin-GOGAT, NADH-GOGAT, NADH-glutamate dehydrogenase and asparagine synthetase. We discerned the specific role of the isoproteins of ferredoxin and ferredoxin:NADP(+) oxidoreductase in providing ferredoxin-GOGAT with photoreduced or enzymatically reduced ferredoxin as the electron donor. The spatial distribution of ferredoxin-GOGAT supported its role in the nitrogen (re)assimilation and reallocation in bundle sheath cells and mesophyll cells of the source leaf. The diurnal nitrogen recycling within the plants took place via the specific amino acids in the phloem and xylem exudates. Taken together, we conclude that the GS1/ferredoxin-GOGAT cycle is the main pathway of inorganic nitrogen assimilation and recycling into glutamine and glutamate, and preconditions amino acid interconversion and remobilization.
Authors:
Marie-Hélène Valadier; Ayako Yoshida; Olivier Grandjean; Halima Morin; Jocelyne Kronenberger; Stéphanie Boutet; Adeline Raballand; Toshiharu Hase; Tadakatsu Yoneyama; Akira Suzuki
Publication Detail:
Type:  Journal Article     Date:  2008-05-08
Journal Detail:
Title:  The FEBS journal     Volume:  275     ISSN:  1742-464X     ISO Abbreviation:  FEBS J.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-23     Completed Date:  2008-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101229646     Medline TA:  FEBS J     Country:  England    
Other Details:
Languages:  eng     Pagination:  3193-206     Citation Subset:  IM    
Affiliation:
Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, Route de St-Cyr, Versailles, France.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Oxidoreductases / analysis
Amino Acids / metabolism*
Biological Transport
Chloroplasts / metabolism
Electron Transport
Gene Expression
Glutamate Synthase / genetics,  metabolism*
Glutamate-Ammonia Ligase / genetics,  metabolism*
Glutamic Acid / biosynthesis
Nitrogen / metabolism
Plant Leaves / cytology,  enzymology,  metabolism
Zea mays / cytology,  enzymology*,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 56-86-0/Glutamic Acid; 7727-37-9/Nitrogen; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.1.13/Glutamate Synthase; EC 1.4.7.1/glutamate synthase (ferredoxin); EC 6.3.1.2/Glutamate-Ammonia Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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