| Implication of ATP and sodium in arachidonic acid incorporation by placental syncytiotrophoblast brush border and basal plasma membranes in the human. | |
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MedLine Citation:
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PMID: 10985969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The human placental syncytiotrophoblast is the main site of exchange of nutrients and minerals between the mother and her fetus. In order to characterize the placental transport of some fatty acids, we studied the incorporation of arachidonic acid, a fetal primordial fatty acid, in purified bipolar syncytiotrophoblast brush border (BBM) and basal plasma membranes (BPM) from human placenta. The basal arachidonic acid incorporation in BBM and BPM was time dependent and reached maximal values of 0.75+/-0.10 and 0.48+/-0.18 pmol/mg protein, respectively, after 2.5 min. The presence of adenosine triphosphate (ATP) (3 m m) increases significantly the maximal incorporation of arachidonic acid by sixfold (4.75+/-0.35 pmol/mg) and ninefold (4.40+/-0.84 pmol/mg) in BBM and BPM, respectively. Moreover, an increase in the arachidonic acid incorporation was also obtained in the presence of sodium where the values achieved 7.68+/-0.98 (10x) and 6.53 pmol/mg (13.6x) for BBM and BPM, respectively. We also showed that the combination of both Na(+)and ATP increases significantly the maximal incorporation of arachidonic acid in BPM to 7.89+/-0.15 pmol/mg protein, while in BBM it did not modify its incorporation (8.18+/-0.25 pmol/mg protein), as compared to the presence of sodium alone. Our results demonstrate that arachidonic acid is incorporated by both placental syncytiotrophoblast membranes, and is ATP and sodium-linked. However, different mechanisms seem to be involved in this fatty acid incorporation through BBM and BPM, since the presence of Na(+)or ATP increased it, while the association of these two elements increased it only in BPM. We also demonstrated by osmolarity experiments that both membranes bind arachidonic acid, potentially involving one or more fatty acids binding proteins. |
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Authors:
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J Lafond; F Moukdar; A Rioux; H Ech-Chadli; L Brissette; J Robidoux; A Masse; L Simoneau |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Placenta Volume: 21 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-10-10 Completed Date: 2001-05-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 661-9 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Harcourt Publishers Ltd. |
Affiliation:
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Laboratoire de Physiologie materno-foetale, Université du Québec à Montréal, Montréal, Canada. lafond.julie@uqam.ca |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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pharmacology* Arachidonic Acid / metabolism* Cell Fractionation Cell Membrane / metabolism* Female Humans Microvilli / metabolism* Osmolar Concentration Pregnancy Sodium / pharmacology* Sucrose / administration & dosage Trophoblasts / ultrastructure* |
| Chemical | |
Reg. No./Substance:
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506-32-1/Arachidonic Acid; 56-65-5/Adenosine Triphosphate; 57-50-1/Sucrose; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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