Document Detail


Implementation of periodic acid-thiosemicarbazide-silver proteinate staining for ultrastructural assessment of muscle glycogen utilization during exercise.
MedLine Citation:
PMID:  2994885     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Distribution of glycogen particles in semithin and ultrathin sections of biopsy samples from human muscles subjected to either short- or long-term running were investigated using PAS and Periodic Acid-ThioSemiCarbazide-Silver Proteinate (PA-TSC-SP) staining methods. Glycogen particles were predominantly found immediately under the sarcolemma or aligned along the myofibrillar I-band. After long-term exhaustive exercise type-1 fibers with a few or no glycogen particles in the core of the fibers were frequently observed. The subsarcolemmal glycogen stores of these "depleted" type-1 fibers were about three times as large as after exhaustive short-time exercise. Another indication of utilization of subsarcolemmal glycogen stores during anaerobic exercise was that many particles displayed a pale, rudimentary shape. This observation suggests fragmental metabolization of glycogen. Thus, depending on type of exercise and type of fiber differential and sequential glycogen utilization patterns can be observed.
Authors:
J Fridén; J Seger; B Ekblom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue research     Volume:  242     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1985  
Date Detail:
Created Date:  1985-11-14     Completed Date:  1985-11-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  229-32     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Glycogen / metabolism*
Histocytochemistry*
Humans
Male
Microscopy, Electron
Muscles / metabolism,  ultrastructure*
Periodic Acid
Periodic Acid-Schiff Reaction*
Physical Exertion*
Running
Semicarbazides
Silver Proteins
Chemical
Reg. No./Substance:
0/Semicarbazides; 0/Silver Proteins; 10450-60-9/Periodic Acid; 7790-28-5/metaperiodate; 79-19-6/thiosemicarbazide; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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