| Implementation of the microdialysis method in the hamster dorsal skinfold chamber. | |
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MedLine Citation:
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PMID: 10639698 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to implement the microdialysis method, a well-established technique for measuring the local concentration of neurotransmitters and metabolites in the brain, in the dorsal skinfold chamber of the awake hamster. First, the effects of implanted, nonperfused microdialysis probes on the microcirculation were examined. Skinfold chambers were prepared with and without probes. Two and 3 days later, the following parameters were assessed: diameter, red blood cell (RBC) velocity, macromolecular leakage, leukocyte rolling fraction, and adherent leukocytes in venules, diameter and macromolecular leakage in arterioles, and functional capillary density (FCD). No significant differences between the animals of the two groups were observed in any of the parameters on either day. Second, the interstitial lactate concentration was measured at two perfusion rates in groups with and without a 4-h tourniquet ischemia. The induction of ischemia resulted in a significant increase in lactate concentration over the control values in the tissue within 1 h to 8000 +/- 860 microM, where it remained until the reperfusion, at which point the concentration returned to control values within 1 h. The microdialysis method provides the opportunity to measure the concentration of metabolites in the extravascular space of the hamster dorsal skinfold chamber. |
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Authors:
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A G Harris; A Schropp; E Schütze; F Krombach; K Messmer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Research in experimental medicine. Zeitschrift für die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie Volume: 199 ISSN: 0300-9130 ISO Abbreviation: Res Exp Med (Berl) Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-02-14 Completed Date: 2000-02-14 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0324736 Medline TA: Res Exp Med (Berl) Country: GERMANY |
Other Details:
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Languages: eng Pagination: 141-52 Citation Subset: IM |
Affiliation:
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Institute for Surgical Research, Klinikum der Ludwig-Maximilians University, Munich, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry Cricetinae Diffusion Chambers, Culture Lactic Acid / analysis Male Mesocricetus Microcirculation / physiology Microdialysis / instrumentation, methods* Neurotransmitter Agents / analysis Reperfusion Injury / metabolism Skin |
| Chemical | |
Reg. No./Substance:
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0/Neurotransmitter Agents; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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