Document Detail


Implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human bladder muscle.
MedLine Citation:
PMID:  8326605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The harvest of human bladder muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human bladder urothelial and muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and bladder muscle cells, when implanted on polyglycolic acid fibers, from new urological structures in vivo composed of both cell types. The human cell-polymer xenografts can be recovered from host animals at extended times after implantation. These data suggest that feasibility of using polyglycolic acid polymers as substrates for the creation of human urothelial and muscle grafts for genitourinary reconstruction.
Authors:
A Atala; M R Freeman; J P Vacanti; J Shepard; A B Retik
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of urology     Volume:  150     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-08-12     Completed Date:  1993-08-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  608-12     Citation Subset:  AIM; IM    
Affiliation:
Pediatric Urology Research Laboratory, Children's Hospital, Boston, Massachusetts.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Epithelial Cells
Epithelium / transplantation
Humans
Mice
Mice, Nude
Muscle, Smooth / cytology,  transplantation
Polyglactin 910*
Prostheses and Implants*
Rabbits
Transplantation, Heterologous
Urinary Bladder / cytology,  transplantation*
Chemical
Reg. No./Substance:
34346-01-5/Polyglactin 910

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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