Document Detail


Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery.
MedLine Citation:
PMID:  24417686     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential of these unique arterial allografts. The 4-mm diameter, 2-3 cm long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000 mmHg and the same compliance as the ovine femoral artery. Grafts were implanted interpositionally in the femoral artery of 6 sheep (n=9), with contralateral sham controls (n=3). At 8 (n=5) and 24 (n=4) wk, all grafts were patent and showed no evidence of dilatation or mineralization. Mid-graft lumen diameter was unchanged. Extensive recellularization occurred, with most cells expressing SMA. Endothelialization was complete by 24 wk with elastin deposition evident. These completely biological grafts possessed circumferential alignment/mechanical anisotropy characteristic of native arteries and were cultured only five weeks prior to decellularization and storage as "off-the-shelf" grafts.
Authors:
Zeeshan Syedain; Lee A Meier; Mathew T Lahti; Sandra Johnson; Robert T Tranquillo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-14
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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