| Imperatorin, a furanocoumarin from Angelica dahurica (Umbelliferae), induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 Cells. | |
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MedLine Citation:
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PMID: 12193260 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Imperatorin, a biologically active furanocoumarin from the roots of Angelica dahurica (Umbelliferae), was found to induce apoptosis in human promyelocytic leukaemia, HL-60 cells. DNA fragmentation assay, morphology-based evaluation, and flow cytometric analysis demonstrated that imperatorin at micromolar concentrations was able to trigger apoptosis of HL-60 cells. Neither necrosis nor differentiation was observed at cytotoxic micromolar concentrations of imperatorin. Further studies showed that the cytochrome c/caspase-9 pathway was responsible for imperatorin-induced apoptosis; i.e., mitochondrial membrane was depolarized, Bcl-2 was down-regulated, cytochrome c was released from mitochondria, caspase-9 and caspase-3 were activated, and poly(ADP-ribose) polymerase was cleaved. Furthermore, imperatorin-induced apoptosis was significantly blocked by Z-VAD-FMK (a broad spectrum caspase inhibitor), Z-LEHD-FMK (a caspase-9 inhibitor) and Ac-DMQD-CHO (a caspase-3 inhibitor), but not by Z-IEDT-FMK (a caspase-8 inhibitor). |
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Authors:
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Hyun-Ock Pae; Hyuncheol Oh; Young-Gab Yun; Gi-Su Oh; Seon Il Jang; Kee-Myoung Hwang; Tae-Oh Kwon; Ho-Sub Lee; Hun-Taeg Chung |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pharmacology & toxicology Volume: 91 ISSN: 0901-9928 ISO Abbreviation: Pharmacol. Toxicol. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-08-23 Completed Date: 2003-04-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8702180 Medline TA: Pharmacol Toxicol Country: Denmark |
Other Details:
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Languages: eng Pagination: 40-8 Citation Subset: IM |
Affiliation:
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Medicinal Resources Research Center of Wonkwang University, Iksan, Chobuk 570-479, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Angelica* Apoptosis* Blotting, Western Caspases / antagonists & inhibitors, metabolism Cell Survival / drug effects Cytochrome c Group / metabolism* Enzyme Activation / drug effects Flow Cytometry HL-60 Cells Humans Mitochondria / drug effects, enzymology, metabolism Psoralens / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Cytochrome c Group; 0/Psoralens; 482-44-0/imperatorin; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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