Document Detail


Impedance sensor technology for cell-based assays in the framework of a high-content screening system.
MedLine Citation:
PMID:  21646704     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Living cultured cells react to external influences, such as pharmaceutical agents, in an intricate manner due to their complex internal signal processing. Impedance sensing of cells on microelectrodes is a favored label-free technology to indicate cellular events, usually ascribed to morphologic alteration or changes in cellular adhesion, which is usually found in stand-alone systems that do not incorporate life support or additional sensor systems. However, only in symbiosis with metabolic activity sensing and picture documentation may a complete insight into cellular vitality be provided. This complement was created within the framework of an automated high-content screening system previously developed by our group, monitoring 24 cell culture chambers in parallel. The objective of this paper is the development of miniaturized electronics for impedance measurements and its system integration as a modular unit. In addition, it is shown how sensor electrodes were optimized by impedance matching such that spectroscopy and raw data analysis become feasible for every culture well. Undesired mechanical stress on cultured cells may arise from the medium and agent support system of the autonomous screening apparatus. This paper demonstrates how this hazard is treated with the simulation of microfluidics and impedance measurements. Physiological data are subsequently derived from the exemplary tumor cell line MCF-7 both during treatment with the agent doxorubicin and through the impact of natural killer cells. This correlates the information content of complex impedance spectra with cellular respiration as well as data from microscopy.
Authors:
T Schwarzenberger; P Wolf; M Brischwein; R Kleinhans; F Demmel; A Lechner; B Becker; B Wolf
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Publication Detail:
Type:  Journal Article     Date:  2011-06-07
Journal Detail:
Title:  Physiological measurement     Volume:  32     ISSN:  1361-6579     ISO Abbreviation:  Physiol Meas     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306921     Medline TA:  Physiol Meas     Country:  England    
Other Details:
Languages:  eng     Pagination:  977-93     Citation Subset:  IM    
Affiliation:
Heinz Nixdorf-Lehrstuhl für Medizinische Elektronik, TU München, Germany.
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