| Impaired response to oxidative stress in senescent cells may lead to accumulation of DNA damage in mesothelial cells from aged donors. | |
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MedLine Citation:
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PMID: 18565326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The accumulation of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) exemplifies oxidative DNA injury, which is strongly implicated in ageing. We show that human peritoneal mesothelial cells (HPMCs) from donors >75 years have lower proliferative capacity but increased 8-OH-dG content compared with cells from individuals <25 years. We detected a positive relationship between the donor's age and the 8-OH-dG level in early-passage HPMCs, and an inverse relationship between those 8-OH-dG levels and subsequent replicative lifespan of HPMCs (n=30). In early-passage cells from donors >75 years, the repair of oxidant-induced 8-OH-dG was delayed compared to cells from donors <25 years. This was coupled with prolonged removal of reactive oxygen species and faster decline in superoxide dismutase activity. Similar effects were observed in HPMCs rendered senescent in vitro. These results indicate that increased 8-OH-dG levels in HPMCs from aged individuals may reflect the in vivo presence of senescent cells with increased vulnerability to oxidative stress-induced DNA damage. |
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Authors:
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Krzysztof Ksiazek; Katarzyna Piatek; Janusz Witowski |
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Publication Detail:
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Type: Journal Article Date: 2008-06-17 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 373 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-07 Completed Date: 2008-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 335-9 Citation Subset: IM |
Affiliation:
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Department of Pathophysiology, Poznan University of Medical Sciences, Swiecickiego 6, 60-781 Poznan, Poland. kksiazek@ump.edu.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aging / genetics, metabolism* Cell Aging* DNA Damage* Deoxyguanosine / analogs & derivatives*, analysis, metabolism Epithelial Cells / cytology, metabolism Female Humans Male Oxidative Stress* Peritoneum / cytology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/8-hydroxy-2'-deoxyguanosine; 961-07-9/Deoxyguanosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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