Document Detail


Impaired response to exercise intervention in the vasculature in metabolic syndrome.
MedLine Citation:
PMID:  23162060     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Physical activity decreases risk for diabetes and cardiovascular disease morbidity and mortality; however, the specific impact of exercise on the diabetic vasculature is unexamined. We hypothesized that an acute, moderate exercise intervention in diabetic and hypertensive rats would induce mitochondrial biogenesis and mitochondrial antioxidant defence to improve vascular resilience. SHHF/Mcc-fa(cp) lean (hypertensive) and obese (hypertensive, insulin resistant), as well as Sprague Dawley (SD) control rats were run on a treadmill for 8 days. In aortic lysates from SD rats, we observed a significant increase in subunit proteins from oxidative phosphorylation (OxPhos) complexes I-III, with no changes in the lean or obese SHHF rats. Exercise also increased the expression of mitochondrial antioxidant defence uncoupling protein 3 (UCP3) (p < 0.05) in SHHF lean rats, whereas no changes were observed in the SD or SHHF obese rats with exercise. We evaluated upstream signalling pathways for mitochondrial biogenesis, and only peroxisome proliferators-activated receptor gamma coactivator 1α (PGC-1α) significantly decreased in SHHF lean rats (p < 0.05) with exercise. In these experiments, we demonstrate absent mitochondrial induction with exercise exposure in models of chronic vascular disease. These findings suggest that chronic vascular stress results in decreased sensitivity of vasculature to the adaptive mitochondrial responses normally induced by exercise.
Authors:
Leslie A Knaub; Sylvia McCune; Adam J Chicco; Matthew Miller; Russell L Moore; Nicholas Birdsey; Monique I Lloyd; Juan Villarreal; Amy C Keller; Peter A Watson; Jane E B Reusch
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-16
Journal Detail:
Title:  Diabetes & vascular disease research : official journal of the International Society of Diabetes and Vascular Disease     Volume:  10     ISSN:  1752-8984     ISO Abbreviation:  Diab Vasc Dis Res     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-10     Completed Date:  2013-10-21     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  101234011     Medline TA:  Diab Vasc Dis Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  222-38     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
AMP-Activated Protein Kinases / metabolism
Animals
Aorta / immunology,  metabolism,  physiopathology
Blood Vessels / immunology,  metabolism,  physiopathology*
Cytokines / blood
Disease Models, Animal*
Hypertension / complications,  metabolism,  physiopathology,  therapy*
Ion Channels / metabolism
Male
Metabolic Syndrome X / etiology,  prevention & control*
Mitochondria / enzymology,  metabolism*
Mitochondrial Proteins / metabolism
Motor Activity*
Nitric Oxide Synthase Type III / metabolism
Obesity / complications,  metabolism,  physiopathology,  therapy*
Oxidative Phosphorylation
Oxidative Stress
RNA-Binding Proteins / metabolism
Rats
Rats, Mutant Strains
Rats, Sprague-Dawley
Transcription Factors / metabolism
Grant Support
ID/Acronym/Agency:
HL14985/HL/NHLBI NIH HHS; K12 HD057022/HD/NICHD NIH HHS; P01 HL014985/HL/NHLBI NIH HHS; R01 DK064741/DK/NIDDK NIH HHS; UL1 RR025780/RR/NCRR NIH HHS; UL1 RR025780/RR/NCRR NIH HHS; UL1 TR001082/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/Ion Channels; 0/Mitochondrial Proteins; 0/Ppargc1a protein, rat; 0/RNA-Binding Proteins; 0/Transcription Factors; 0/mitochondrial uncoupling protein 3; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, rat; EC 2.7.11.1/AMP-Activated Protein Kinases
Comments/Corrections

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