| Impaired nociception and peripheral opioid antinociception in mice lacking both kinin b1 and b2 receptors. | |
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MedLine Citation:
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PMID: 22273860 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: : Kinins (e.g., bradykinin) acting through the constitutively expressed B2 and the injury-induced B1 receptors are involved in pain and hyperalgesia, as previously shown by use of receptor-selective antagonists and single-receptor knockout models. Because the overall contribution of kinins to painful processes remains unclear, the aim of this study was to analyze pain-related behaviors of mice unable to respond to kinins because of a lack of both B1 and B2 receptors. METHODS: : In knockout mice lacking both B1 and B2 receptors and in wild-type mice (n = 8-21 per group) the authors assessed nociceptive thresholds to mechanical and heat stimuli (von Frey and Hargreaves tests, respectively) in healthy animals and after induction of inflammatory and neuropathic pain, acid-induced visceral nociception, and modulation of nociceptive responses by peripherally administered opioid agonists. RESULTS: : In knockout mice lacking both B1 and B2 receptors baseline nociceptive responses to heat were unaltered, nocifensive responses to bradykinin were abolished, acute acetic acid-induced visceral nociception was reduced by approximately 70% (mean difference: 19.5 writhes/30 min) and heat hypersensitivity in carrageenan-induced paw inflammation was decreased 48 h after injection (mean difference 2.88 s), hypersensitivities in chronic complete Freund's adjuvant-induced paw inflammation or after chronic constriction injury of the sciatic nerve were unchanged, and peripheral μ- and δ-opioid-induced analgesia after chronic constriction injury was reduced by 30-35% (mean differences: μ-agonist: 0.495 g, δ-agonist: 0.555 g). CONCLUSIONS: : These data suggest that kinins are important for nociception associated with acute short-lasting inflammation but are less essential in chronic stages of pain. The results also highlight a new protective function of kinins via interactions with the opioid system. |
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Authors:
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Cecile Cayla; Dominika Labuz; Halina Machelska; Michael Bader; Michael Schäfer; Christoph Stein |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anesthesiology Volume: 116 ISSN: 1528-1175 ISO Abbreviation: Anesthesiology Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1300217 Medline TA: Anesthesiology Country: United States |
Other Details:
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Languages: eng Pagination: 448-57 Citation Subset: AIM; IM |
Affiliation:
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* Principal Scientist, † Scientist, ‡ Professor, # Chair, Klinik für Anaesthesiologie und Operative Intensivmedizin, Campus Benjamin Franklin, Berlin, Germany. § Professor, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany. ‖ Current position: Professor, Campus Virchow Klinikum, Charité-Universitätsmedizin, Berlin, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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