| Impaired macrophage phagocytosis in non-eosinophilic asthma. | |
| | |
MedLine Citation:
|
PMID: 23278878 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
BACKGROUND: Many patients with non-eosinophilic asthma have increased numbers of neutrophils in the airways. The explanation for this chronic inflammation remains unclear, but may result from an impaired ability of alveolar macrophages to phagocytose apoptotic cells (a process termed 'efferocytosis'), as we have shown in chronic obstructive pulmonary disease (COPD). OBJECTIVES: To examine induced sputum as a non-invasive technique to characterize efferocytosis in chronic lung diseases and to compare efferocytosis in patients with non-eosinophilic asthma, eosinophilic asthma and COPD. METHODS: Participants with stable asthma (20 with eosinophilic and 30 with non-eosinophilic) and COPD (n = 11) underwent clinical assessment including allergy skin tests, saline challenge and sputum induction. Sputum cells were dispersed using dithiothreitol and resuspended in culture medium. Efferocytosis of apoptotic bronchial epithelial cells by sputum-derived macrophages was determined using flow cytometry. RESULTS: There were no significant differences in efferocytosis between paired sputum and bronchoalveolar lavage macrophages from three subjects. Efferocytosis was significantly impaired in patients with non-eosinophilic asthma [mean (SD) 0.95 (0.24)] compared with eosinophilic asthma [1.17 (0.19)] and to a similar degree as patients with COPD [1.04 (0.16)]. Sputum neutrophils were significantly higher in patients with COPD and non-eosinophilic asthma compared with eosinophilic asthma. CONCLUSION AND CLINICAL RELEVANCE: Induced sputum provides a reliable and non-invasive method for studying macrophage efferocytosis in chronic lung disease. Macrophage efferocytosis is impaired in non-eosinophilic asthma to a similar degree as that in COPD and may explain the persistent airway neutrophilia and chronic inflammation that characterizes this asthma subtype. |
| | |
Authors:
|
J L Simpson; P G Gibson; I A Yang; J Upham; A James; P N Reynolds; S Hodge; |
Related Documents
:
|
2191058 - The biology of the t-cell antigen receptor and its role in the skin immune system. 18573908 - Foxp3+ regulatory t cells maintain immune homeostasis in the skin. 15787618 - Harnessing wound healing and regeneration for tissue engineering. 2759658 - Flare-up reaction on murine contact hypersensitivity. i. description of an experimental... 22819828 - Immunoregulatory molecules are master regulators of inflammation during the immune resp... 23561928 - Nucleosomes contribute to increase mesangial cell chemokine expression during the devel... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 43 ISSN: 1365-2222 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-01-02 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: England |
Other Details:
|
Languages: eng Pagination: 29-35 Citation Subset: IM |
Copyright Information:
|
© 2012 Blackwell Publishing Ltd. |
Affiliation:
|
Centre for Asthma and Respiratory Disease, The University of Newcastle, Newcastle, Australia; Department of Respiratory and Sleep Medicine, Hunter Medical Research Institute, Newcastle, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Adherence to pharmacological treatment and specific immunotherapy in allergic rhinitis.
Next Document: Dietary restriction and exercise improve airway inflammation and clinical outcomes in overweight and...