| Impaired lipid accumulation by trans10, cis12 CLA during adipocyte differentiation is dependent on timing and length of treatment. | |
| | |
MedLine Citation:
|
PMID: 15708349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Conjugated linoleic acids (CLAs) are a group of polyunsaturated fatty acids found in ruminant products, where the predominant isomers are cis9, trans11 (c9,t11) and trans10, cis12 (t10,c12) CLA. We have previously shown that t10,c12 CLA prevents lipid accumulation in mature adipocytes in part by acting as a peroxisome proliferator-activated receptor gamma (PPAR gamma) modulator. The objective of this study was to further establish the molecular mechanisms underlying the attenuating effect on lipid accumulation by t10,c12 CLA, with focus on time point and duration of treatment during adipogenesis. We have shown that t10,c12 CLA treatment has its most attenuating effect early (day (D) 0-6) during differentiation. Treatment during this period is sufficient to prevent lipid accumulation in mature adipocytes. The adipogenic marker genes PPAR gamma and CCAAT/enhancer binding protein alpha (C/EBP alpha) are both down-regulated after treatment within the period from D0-6, while additional treatment also down-regulates the expression of sterol regulatory element binding protein-1c (SREBP-1c), liver X receptor alpha (LXR alpha), fatty acid binding protein (aP2), fatty acid translocase (CD36) and insulin-sensitive glucose transporter 4 (GLUT4). These effects of t10,c12 CLA reflect the subsequent attenuation of lipid accumulation observed in mature adipocytes. Interestingly, the early B-cell factor (O/E-1), which is known to promote adipogenesis and to be involved in control of genes important for terminal adipocyte differentiation, is unaffected by treatment of t10,c12 CLA. Taken together, our data indicate that inhibition of lipid accumulation induced by t10,c12 CLA treatment during adipocyte differentiation is associated with a tight regulatory cross-talk between early (PPAR gamma and C/EBP alpha) and late (LXR alpha, aP2 and CD36) adipogenic marker genes. |
| | |
Authors:
|
Linda Granlund; Jan I Pedersen; Hilde I Nebb |
Related Documents
:
|
11510559 - Separation of glyceride positional isomers by silver ion chromatography. 3236079 - Formation of a biotin precursor, pimelic acid, in yeasts from c18 fatty acids. 7213499 - Effect of actinomycin d on serum betalipoproteins of partially hepatectomized dogs. iii... 17209019 - Metabolism of linoleic acid by human gut bacteria: different routes for biosynthesis of... 20509029 - On mechanism of quorum sensing in candida albicans by 3(r)-hydroxy-tetradecaenoic acid. 12843679 - Purification of conjugated linoleic acid isomers through a process including lipase-cat... 464689 - Evidence for a pyloro-cholecystic reflex for gallbladder contraction. 22125139 - New advances in amino acid profiling by capillary electrophoresis-electrospray ionizati... 17178719 - Functional characterization of desaturases involved in the formation of the terminal do... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1687 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2005 Feb |
Date Detail:
|
Created Date: 2005-02-14 Completed Date: 2005-04-18 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 11-22 Citation Subset: IM |
Affiliation:
|
Institute of Basic Medical Sciences, Department of Nutrition, University of Oslo, N-0316 Oslo, Norway. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3T3 Cells Adipocytes / cytology, drug effects*, physiology* Animals Antigens, CD36 / genetics, metabolism CCAAT-Enhancer-Binding Protein-alpha / genetics, metabolism CCAAT-Enhancer-Binding Proteins / genetics, metabolism Carrier Proteins / genetics, metabolism Cell Differentiation / physiology* DNA-Binding Proteins / genetics, metabolism Fatty Acid-Binding Proteins Gene Expression Regulation, Developmental* Glucose Transporter Type 4 Humans Linoleic Acids, Conjugated / pharmacology* Lipid Metabolism* Mice Monosaccharide Transport Proteins / genetics, metabolism Muscle Proteins / genetics, metabolism Orphan Nuclear Receptors PPAR gamma / genetics, metabolism Receptors, Cytoplasmic and Nuclear / genetics, metabolism Signal Transduction / physiology Sterol Regulatory Element Binding Protein 1 Time Factors Transcription Factors / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD36; 0/CCAAT-Enhancer-Binding Protein-alpha; 0/CCAAT-Enhancer-Binding Proteins; 0/Carrier Proteins; 0/DNA-Binding Proteins; 0/Fatty Acid-Binding Proteins; 0/Glucose Transporter Type 4; 0/Linoleic Acids, Conjugated; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/Orphan Nuclear Receptors; 0/PPAR gamma; 0/Receptors, Cytoplasmic and Nuclear; 0/SLC2A4 protein, human; 0/SREBF1 protein, human; 0/Slc2a4 protein, mouse; 0/Srebf1 protein, mouse; 0/Sterol Regulatory Element Binding Protein 1; 0/Transcription Factors; 0/liver X receptor; 0/trans-10,cis-12-conjugated linoleic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The apolipoprotein(a) component of lipoprotein(a) mediates binding to laminin: contribution to selec...
Next Document: Effects of structural changes of fatty acids on lipid accumulation in adipocytes and primary hepatoc...