Document Detail

Impaired insulin secretion in perfused pancreases isolated from offspring of female rats fed a low protein whey-based diet.
MedLine Citation:
PMID:  18648139     Owner:  NLM     Status:  MEDLINE    
CONTEXT: Insufficient maternal protein intake has been postulated to cause impaired fuel metabolism and diabetes mellitus in adult mammalian progeny, but the mechanism remains unclear. OBJECTIVE: To investigate the effect of a maternal low protein whey-based diet during pregnancy and lactation on pancreatic function and skeletal muscle glucose metabolism in the offspring. ANIMALS: Sprague-Dawley rats: 8 mothers and 46 offspring. DESIGN: Female rats were fed throughout pregnancy and lactation with otherwise-complete isoenergetic diets sufficient (20% whey protein; control: n=3) or insufficient (5% whey protein; low-protein: n=5) in whey protein. From weaning all offspring ate control diet. MAIN OUTCOME MEASURES: Food intake and weight gain were measured for both mothers and offspring, and in vitro functional studies of endocrine pancreas and skeletal muscle were performed on offspring at 40 and 50 days of age, respectively. RESULTS: Food intake (P=0.004) and weight gain (P=0.006) were lower in low protein than control mothers during early gestation. Offspring of low protein mothers had significant lower body weight from 5 to 15 days of age, although there was no significant difference in food consumption. Glucose, arginine- and glucose/arginine-stimulated insulin secretion from perfused pancreases isolated from low protein offspring were decreased by between 55 and 65% compared with control values. Studies in skeletal muscle demonstrated no difference in insulin sensitivity between the two groups. CONCLUSIONS: Dietary whey protein insufficiency in female rats during pregnancy and lactation can evoke major changes in insulin secretion in progeny, and these changes represent a persistent functional abnormality in the endocrine pancreas.
Matthew P G Barnett; Anthony R J Phillips; Patricia M Harris; Garth J S Cooper
Related Documents :
20507349 - Dietary exposure to low doses of bisphenol a: effects on reproduction and development i...
4049399 - Toxicity of gestational aluminum exposure to the maternal rabbit and offspring.
24792809 - Effects of dietary amylase and sucrose on productivity of cows fed low-starch diets.
24778329 - Evaluation of saccharomyces cerevisiae fermentation product as an alternative to monens...
9566509 - The fatty acid composition of maternal diet affects the response to excitotoxic neural ...
16419579 - Studies of the toxicological potential of tripeptides (l-valyl-l-prolyl-l-proline and l...
22443629 - Iodine and selenium carry over in milk and cheese in dairy cows: effect of diet supplem...
23750829 - Review article socio-economic determinants of micronutrient intake and status in europe...
7265309 - Effects of chronic ingestion of no. 2 fuel oil on mallard ducklings.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-10
Journal Detail:
Title:  JOP : Journal of the pancreas     Volume:  9     ISSN:  1590-8577     ISO Abbreviation:  JOP     Publication Date:  2008  
Date Detail:
Created Date:  2008-07-23     Completed Date:  2008-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101091810     Medline TA:  JOP     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  477-88     Citation Subset:  IM    
School of Biological Sciences, Faculty of Science, The University of Auckland, Auckland, New Zealand.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Newborn
Blood Glucose
Body Weight
Diet, Protein-Restricted* / adverse effects
Glucose / metabolism,  pharmacokinetics
Glycogen / metabolism
Insulin / biosynthesis,  pharmacology,  secretion*
Lactation / metabolism
Milk Proteins / adverse effects*
Muscle, Skeletal / drug effects,  metabolism
Organ Culture Techniques
Pancreas / metabolism,  secretion*
Prenatal Exposure Delayed Effects
Rats, Sprague-Dawley
Weight Gain
Reg. No./Substance:
0/Blood Glucose; 0/Milk Proteins; 0/whey protein; 11061-68-0/Insulin; 50-99-7/Glucose; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The impact of obesity on surgical outcome after pancreaticoduodenectomy.
Next Document:  Prevalence of fibrocalculous pancreatic diabetes in Chennai in South India.