| Impaired glucose tolerance plus hyperlipidaemia induced by diet promotes retina microaneurysms in New Zealand rabbits. | |
| | |
MedLine Citation:
|
PMID: 21272105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
With the increasing prevalence of diabetes mellitus and metabolic syndrome worldwide, experimental models are required to better understand the pathophysiology and therapeutic approaches to preserve pancreatic beta cells, attenuate atherosclerosis and protect target organs. The aims of this study were to develop an experimental model of impaired glucose tolerance combined with hypercholesterolaemia induced by diet and assess metabolic alterations and target organ lesions. New Zealand male rabbits were fed high-fat/high-sucrose (10/40%) and cholesterol-enriched diet for 24 weeks, when they were sacrificed. Biochemistry, fundus photographs with fluorescein angiography and pathological analyses were performed. Cholesterol-fed and normal animals of same age were compared. Results: The animals with diet-induced impaired glucose tolerance combined with hypercholesterolaemia gained weight, increased blood glucose, total cholesterol, LDL-C and triglycerides and decreased HDL-C (P < 0.05 vs. baseline). Fructosamine levels and the homeostasis model assessment of insulin resistance (HOMA-IR) index were increased, while there was a reduction in the HOMA-β (P < 0.05 for all vs. baseline). Histomorphologic findings of this model were aortic atherosclerosis, hepatic steatofibrosis and glomerular macrophage infiltration. Early clinical features of diabetic retinopathy with hyperfluorescent dots consistent with presence of retina microaneurysms were seen since week 12, progressing up to the end of the experiment (P < 0.0005 vs. baseline and 12 weeks). Our model reproduced several metabolic characteristics of human diabetes mellitus and promoted early signs of retinopathy. This non-expensive model is suitable for studying mechanistic pathways and allowing novel strategic approaches. |
| | |
Authors:
|
Tatiana Helfenstein; Francisco A Fonseca; Sílvia S Ihara; Juliana M Bottós; Flávio T Moreira; Henrique Pott; Michel E Farah; Maria C Martins; Maria C Izar |
Related Documents
:
|
682055 - Riboflavin requirement of channel catfish fingerlings. 6941675 - The acute inflammatory process in copper-deprived rats. 3975225 - Effects of different dietary carbohydrates on hepatic enzymes of copper-deficient rats. 3764095 - Accumulation of copper on albumin in bovine plasma in vivo after intravenous trithiomol... 18936215 - Methionine-adequate cysteine-free diet does not limit erythrocyte glutathione synthesis... 8862485 - Antioxidant vitamins in hospitalized elderly patients: analysed dietary intakes and bio... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: International journal of experimental pathology Volume: 92 ISSN: 1365-2613 ISO Abbreviation: Int J Exp Pathol Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-01-28 Completed Date: 2011-03-14 Revised Date: 2012-02-01 |
Medline Journal Info:
|
Nlm Unique ID: 9014042 Medline TA: Int J Exp Pathol Country: England |
Other Details:
|
Languages: eng Pagination: 40-9 Citation Subset: IM |
Copyright Information:
|
© 2011 The Authors. International Journal of Experimental Pathology © 2011 International Journal of Experimental Pathology. |
Affiliation:
|
Cardiology Division, Department of Medicine, Federal University of Sao Paulo, Sao Paulo, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aneurysm
/
etiology,
pathology*,
physiopathology Animals Aorta / pathology Atherosclerosis / etiology, pathology, physiopathology Diabetes Mellitus, Type 2 / complications, pathology, physiopathology* Diabetic Retinopathy / etiology, pathology*, physiopathology Diet Fatty Liver / etiology, pathology, physiopathology Glucose Intolerance / complications, pathology, physiopathology* Hyperlipidemias / complications, pathology, physiopathology* Immunohistochemistry Male Rabbits Retina / pathology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ischaemia-reperfusion injury in orthotopic mouse lung transplants - a scanning electron microscopy s...
Next Document: Fifteen-year follow-up of thyroid status in adults with Down syndrome.