Document Detail


Impaired gluconeogenesis in a porcine model of paracetamol induced acute liver failure.
MedLine Citation:
PMID:  21472104     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF).
METHODS: Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using (1)H NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples.
RESULTS: No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05).
CONCLUSION: Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state.
Authors:
Konstantinos J Dabos; Henry R Whalen; Philip N Newsome; John A Parkinson; Neil C Henderson; Ian H Sadler; Peter C Hayes; John N Plevris
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  17     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-04-07     Completed Date:  2011-07-25     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  1457-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetaminophen
Amino Acids / blood
Animals
Biological Markers / blood
Citric Acid Cycle
Disease Models, Animal
Gluconeogenesis*
Glycolysis
Lactic Acid / blood
Liver / metabolism*
Liver Failure, Acute / chemically induced,  metabolism*
Magnetic Resonance Spectroscopy
Pyruvic Acid / blood
Swine
Time Factors
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Biological Markers; 33X04XA5AT/Lactic Acid; 362O9ITL9D/Acetaminophen; 8558G7RUTR/Pyruvic Acid
Comments/Corrections

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