| Impaired gluconeogenesis in a porcine model of paracetamol induced acute liver failure. | |
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MedLine Citation:
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PMID: 21472104 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF). METHODS: Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using (1)H NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples. RESULTS: No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05). CONCLUSION: Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state. |
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Authors:
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Konstantinos J Dabos; Henry R Whalen; Philip N Newsome; John A Parkinson; Neil C Henderson; Ian H Sadler; Peter C Hayes; John N Plevris |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 17 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-04-07 Completed Date: 2011-07-25 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 1457-61 Citation Subset: IM |
Affiliation:
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Liver Cell Biology Laboratory, Department of Hepatology, University of Edinburgh, Edinburgh, EH16 4SA, United Kingdom. kostasophia@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetaminophen Amino Acids / blood Animals Biological Markers / blood Citric Acid Cycle Disease Models, Animal Gluconeogenesis* Glycolysis Lactic Acid / blood Liver / metabolism* Liver Failure, Acute / chemically induced, metabolism* Magnetic Resonance Spectroscopy Pyruvic Acid / blood Swine Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Biological Markers; 103-90-2/Acetaminophen; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid |
| Comments/Corrections | |
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