Document Detail


Impaired fatty acid oxidation in a Drosophila model of mitochondrial trifunctional protein (MTP) deficiency.
MedLine Citation:
PMID:  22342726     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mitochondrial trifunctional protein (MTP), which consists of the MTPα and MTPβ subunits, catalyzes long-chain fatty acid β-oxidation. MTP deficiency in humans results in Reye-like syndrome. Here, we generated Drosophila models of MTP deficiency by targeting two genes encoding Drosophila homologs of human MTPα and MTPβ, respectively. Both Mtpα(KO) and Mtpβ(KO) flies were viable, but demonstrated reduced lifespan, defective locomotor activity, and reduced fecundity represented by the number of eggs laid by the females. The phenotypes of Mtpα(KO) flies were generally more striking than those of Mtpβ(KO) flies. Mtpα(KO) flies were hypersensitive to fasting, and retained lipid droplets in their fat body cells as in non-fasting conditions. The amount of triglyceride was also unchanged upon fasting in Mtpα(KO) flies, suggesting that lipid mobilization was disrupted. Finally, we showed that both Mtpα(KO) and Mtpβ(KO) flies accumulated acylcarnitine and hydroxyacylcarnitine, diagnostic markers of MTP deficiencies in humans. Our results indicated that both Mtpα(KO) and Mtpβ(KO) flies were impaired in long-chain fatty acid β-oxidation. These flies should be useful as a model system to investigate the molecular pathogenesis of MTP deficiency.
Authors:
Yoshihito Kishita; Manabu Tsuda; Toshiro Aigaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-10
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  419     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-12     Completed Date:  2012-05-07     Revised Date:  2012-11-16    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  344-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji, Tokyo 192-0397, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster / enzymology*,  genetics
Fatty Acids / metabolism*
Gene Knockout Techniques
Mitochondria / enzymology*
Models, Animal
Multienzyme Complexes / genetics,  metabolism*
Oxidation-Reduction
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Fatty Acids; 0/Multienzyme Complexes; 0/mitochondrial fatty acid beta-oxidation trifunctional protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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