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Impaired bacillus calmette-Guérin cellular immune response in Human Immunodeficiency Virus-exposed, uninfected infants.
MedLine Citation:
PMID:  21866040     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE:: To evaluate cell-mediated immune response to Bacillus Calmette-Guérin (BCG) vaccination in uninfected, HIV-1-exposed infants, comparing it to unexposed children. DESIGN:: Cross-sectional study. METHODS:: BCG-specific lymphoproliferation and T cell subsets (CD4, CD8 and TCR γδ) by flow cytometry and IL-10, IFN-γ and TNF-α concentration by ELISA were analyzed in HIV-exposed and unexposed infants. Whole blood lymphocyte immunophenotyping and blood counts were performed in exposed children. Nonparametric tests were used (P < 0.05). RESULTS:: Given the ontogeny of the immune system, exposed infants were separated into three groups, according to age: Exposed 1 (E1, aged 6.1-8.8), E2 (aged 9.1-17.1) and E3 (aged 18.1-26.3). Unexposed infants (UE) and E1 were matched for age. Cell proliferation was not different among the three exposed groups, neither for BCG nor for PHA-stimulated cultures. Furthermore, BCG-stimulated lymphoproliferation was reduced in the E1 group in comparison to UE. T lymphocyte subpopulations also showed differences, with the youngest HIV-exposed groups (E1 and E2) showing a predominant proliferation of CD4 T cells in cultures with BCG, while E3 and UE had a robust γδ T cell expansion. There was lower IFN-γ concentration in the samples from E1 in comparison to all of the other groups. The unexposed infants showed higher TNF-α concentration in cultures with BCG and PHA in comparison to E1. CONCLUSIONS:: BCG-specific T cell proliferation was reduced in HIV-exposed uninfected infants and IFN-γ concentration was lower in younger exposed infants, showing a delay in immune system maturation of HIV-exposed infants.
Authors:
Taís N Mazzola; Marcos T N da Silva; Beatriz M Abramczuk; Yara M F Moreno; Simone C B S Lima; Tatiane Q Zorzeto; Andréa S Z Passeto; Maria M S Vilela
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-23
Journal Detail:
Title:  AIDS (London, England)     Volume:  -     ISSN:  1473-5571     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8710219     Medline TA:  AIDS     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
aCenter for Pediatrics Investigation (CIPED), Medical Faculty, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil bCurrent adress: Nutrition Department, Health Sciences Centre, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
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