Document Detail

Impaired interleukin-8 chemokine secretion by staphylococcus aureus-activated epithelium and T-cell chemotaxis in cystic fibrosis.
MedLine Citation:
PMID:  19597126     Owner:  NLM     Status:  MEDLINE    
Staphylococcus aureus is frequently isolated from lungs of patients with cystic fibrosis (CF). Upon lung infection with S. aureus, airway epithelial cells (AEC) produce high levels of chemokines that enhance T-cell chemotaxis. Although the number of lymphocytes is increased in the airways and bronchoalveolar lavage fluid of patients with CF, the mechanisms responsible for their accumulation and the role of S. aureus in this process are largely unknown. This study investigated early S. aureus impact on chemokine secretion by CF epithelial cells and chemotaxis of CF T cells. CF and non-CF AEC were grown in a cell culture model and apically stimulated with S. aureus. Supernatants were quantified for chemokine secretions and assayed for T-cell chemotaxis. CF AEC secreted constitutively larger amounts of IL-8, GROalpha, MIG, MIP-3beta, and MCP-1 than non-CF epithelial cells. S. aureus interaction with epithelial cells increased chemokine production by non-CF cells but had no effect on CF cells. Chemotaxis of T cells derived from patients with CF was greater than that of T cells from subjects without CF. Moreover, there were more CF T cells expressing CXCR1 as compared with non-CF T cells. Under our experimental conditions, inhibition of IL-8 or its receptor CXCR1 resulted in a considerable decrease in T-cell chemotaxis (up to 80%). These data suggest that IL-8 and its receptor CXCR1 are key players in the chemotaxis of CF T cells and could be used as targets to develop therapies for CF.
Denise Al Alam; Gaetan Deslee; Claire Tournois; Bouchaib Lamkhioued; Fran?ois Lebargy; Marc Merten; Azzaq Belaaouaj; Moncef Guenounou; Sophie Catherine Gangloff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-13
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  42     ISSN:  1535-4989     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-28     Completed Date:  2010-06-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  644-50     Citation Subset:  IM    
Laboratoire d'Immunologie et de Microbiologie EA3796, IFR53, UFR de Pharmacie 1 avenue du Mar?chal Juin 51100 Reims, France.
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MeSH Terms
Antibodies, Monoclonal
Antigens, CD3 / immunology
Case-Control Studies
Cell Line
Chemotaxis, Leukocyte*
Cystic Fibrosis / immunology*,  microbiology
Electric Impedance
Interleukin-8 / immunology*,  secretion
Receptors, Interleukin-8A / immunology
Recombinant Proteins / immunology
Respiratory Mucosa / immunology*,  microbiology,  secretion
Staphylococcus aureus / pathogenicity*
T-Lymphocytes / immunology*,  microbiology
Time Factors
Young Adult
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/IL8 protein, human; 0/Interleukin-8; 0/Receptors, Interleukin-8A; 0/Recombinant Proteins

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