| Impacts of dietary selenium deficiency on metabolic phenotypes of diet-restricted GPX1-overexpressing mice. | |
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MedLine Citation:
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PMID: 20578960 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously reported a spontaneous development of type 2 diabetes-like phenotypes in glutathione peroxidase-1 (GPX1)-overexpressing (OE) mice. Diet restriction of these mice rescued all their phenotypes, except for hyperinsulinemia and hypersecretion of insulin. This study was to determine whether dietary Se deficiency eliminated these two primary effects of GPX1 overproduction. Forty-seven male OE and wild-type (WT) mice were fed an Se-adequate (0.4 mg Se/kg) or deficient (<0.02 mg Se/kg) diet at 2 to 3 g (full-fed = 5 g) per day from 4 to 12 weeks of age. Although dietary Se deficiency did not rescue the primary phenotypes of the diet-restricted OE mice, it exerted a strong effect (p < 0.05) on mRNA or protein levels (or both) of 14 molecules involved in islet insulin synthesis and secretion and hepatic lipogenesis. Dietary Se deficiency exhibited a hypoinsulinemic trend in OE mice and a strong hypolipidemic effect (p < 0.05) in the liver of WT mice. Hepatic lipogenesis was attenuated in OE compared with WT mice. In conclusion, diet restriction might be too overwhelming to allow a demonstration of a dietary Se-depletion effect on the OE phenotypes. Full-fed animals could offer a better chance to illustrate such effects and the underlying mechanisms. |
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Authors:
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Matthew P Pepper; Marko Z Vatamaniuk; Xi Yan; Carol A Roneker; Xin Gen Lei |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-29 |
Journal Detail:
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Title: Antioxidants & redox signaling Volume: 14 ISSN: 1557-7716 ISO Abbreviation: Antioxid. Redox Signal. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-05 Completed Date: 2011-04-14 Revised Date: 2012-02-01 |
Medline Journal Info:
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Nlm Unique ID: 100888899 Medline TA: Antioxid Redox Signal Country: United States |
Other Details:
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Languages: eng Pagination: 383-90 Citation Subset: IM |
Affiliation:
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Department of Animal Science, Cornell University, Ithaca, New York 14853, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caloric Restriction* Diabetes Mellitus, Type 2 / metabolism Diet* Energy Metabolism* Glutathione Peroxidase / genetics, metabolism* Islets of Langerhans / physiology Liver / physiology Male Mice Mice, Inbred C3H Mice, Inbred C57BL Phenotype* Selenium / deficiency* |
| Grant Support | |
ID/Acronym/Agency:
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DK53018/DK/NIDDK NIH HHS; R01 DK053018-11/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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7782-49-2/Selenium; EC 1.11.1.-/glutathione peroxidase GPX1; EC 1.11.1.9/Glutathione Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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