Document Detail


Impact of warm ischemic time on microsomal P450 isoforms in a porcine model of therapeutic liver resection.
MedLine Citation:
PMID:  15501478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human microsomes and hepatocytes obtained from non-transplantable livers of brain-dead donors are very useful in predicting the in vivo metabolism of xenobiotics in humans. Fresh liver specimens obtained from therapeutic liver resection are also useful for research in cases where non-transplantable livers are not readily available. In the present study, the effect of warm ischemic duration, in the course of hepatic surgery, on the activities of liver cytochrome P450 (CYP) CYP1A, CYP2C, CYP2D, CYP2E1 and CYP3A were evaluated in a porcine model. Partial occlusion (portal vein and hepatic artery occlusion) decreased the activities of CYP2C, CYP2E and CYP3A, but not those of CYP1A and CYP2D. CYP3A, known to account for an average 30% of total P450 content in the human liver was the most susceptible to the warm ischemia. These results demonstrate that the activities of CYP isoforms, particularly those of CYP3A, are markedly affected by warm ischemia; it is, therefore, essential that care should be exercised when using microsomes prepared from surgically removed livers.
Authors:
Satoshi Suzuki; Tetsuo Satoh; Hiroyuki Yoshino; Eiji Kobayashi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  76     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-25     Completed Date:  2004-12-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  39-46     Citation Subset:  IM    
Affiliation:
Research Laboratories, HAB Research Organization, Cornea Center Bldg. 3rd Floor, Ichikawa General Hospital, 5-11-13 Sugano, Ichikawa, Chiba 272-8523, Japan. suzuki@hab.or.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cytochrome P-450 Enzyme System / metabolism*
Hot Temperature*
Immunoblotting
Ischemia / metabolism,  physiopathology*
Isoenzymes / metabolism
Liver / metabolism*,  surgery
Microsomes, Liver / metabolism*
Models, Animal
Sus scrofa
Chemical
Reg. No./Substance:
0/Isoenzymes; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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