| Impact of warm ischemic time on microsomal P450 isoforms in a porcine model of therapeutic liver resection. | |
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MedLine Citation:
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PMID: 15501478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human microsomes and hepatocytes obtained from non-transplantable livers of brain-dead donors are very useful in predicting the in vivo metabolism of xenobiotics in humans. Fresh liver specimens obtained from therapeutic liver resection are also useful for research in cases where non-transplantable livers are not readily available. In the present study, the effect of warm ischemic duration, in the course of hepatic surgery, on the activities of liver cytochrome P450 (CYP) CYP1A, CYP2C, CYP2D, CYP2E1 and CYP3A were evaluated in a porcine model. Partial occlusion (portal vein and hepatic artery occlusion) decreased the activities of CYP2C, CYP2E and CYP3A, but not those of CYP1A and CYP2D. CYP3A, known to account for an average 30% of total P450 content in the human liver was the most susceptible to the warm ischemia. These results demonstrate that the activities of CYP isoforms, particularly those of CYP3A, are markedly affected by warm ischemia; it is, therefore, essential that care should be exercised when using microsomes prepared from surgically removed livers. |
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Authors:
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Satoshi Suzuki; Tetsuo Satoh; Hiroyuki Yoshino; Eiji Kobayashi |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Life sciences Volume: 76 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-10-25 Completed Date: 2004-12-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
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Languages: eng Pagination: 39-46 Citation Subset: IM |
Affiliation:
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Research Laboratories, HAB Research Organization, Cornea Center Bldg. 3rd Floor, Ichikawa General Hospital, 5-11-13 Sugano, Ichikawa, Chiba 272-8523, Japan. suzuki@hab.or.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cytochrome P-450 Enzyme System / metabolism* Hot Temperature* Immunoblotting Ischemia / metabolism, physiopathology* Isoenzymes / metabolism Liver / metabolism*, surgery Microsomes, Liver / metabolism* Models, Animal Sus scrofa |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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