Document Detail


Impact of thiamine deficiency on T-cell dependent and T-cell independent antibody production in lake trout.
MedLine Citation:
PMID:  23134222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lake trout Salvelinus namaycush on thiamine-replete and thiamine-depleted diets were evaluated for the effects of thiamine status on in vivo responses to the T-dependent antigen trinitophenol (TNP)-keyhole limpet hemocyanin (TNP-KLH), the T-independent antigen trinitrophenol-lipolysaccaharide (TNP-LPS), or Dulbecco's phosphate-buffered saline (DPBS; negative control fish). Plasma antibody concentrations were evaluated for possible differences in total anti-TNP activity as well as differences in response kinetics. Associations between anti-TNP activity and muscle and liver thiamine concentrations as well as ratios of muscle-to-liver thiamine to anti-TNP activity were also examined. Thiamine-depleted lake trout that were injected with TNP-LPS exhibited significantly more anti-TNP activity than thiamine-replete fish. The depleted fish injected with TNP-LPS also exhibited significantly different response kinetics relative to thiamine-replete lake trout. No differences in activity or kinetics were observed between the thiamine-replete and -depleted fish injected with TNP-KLH or in the DPBS negative controls. Anti-TNP activity in thiamine-depleted lake trout injected with TNP-KLH was positively associated with muscle thiamine pyrophosphate (thiamine diphosphate; TPP) concentration. A negative association was observed between the ratio of muscle-to-liver TPP and T-independent responses. No significant associations between anti-TNP activity and tissue thiamine concentration were observed in the thiamine-replete fish. We demonstrated that thiamine deficiency leads to alterations in both T-dependent and T-independent immune responses in lake trout.
Authors:
Christopher A Ottinger; Dale C Honeyfield; Christine L Densmore; Luke R Iwanowicz
Publication Detail:
Type:  Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of aquatic animal health     Volume:  24     ISSN:  0899-7659     ISO Abbreviation:  J. Aquat. Anim. Health     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-08     Completed Date:  2013-03-14     Revised Date:  2013-11-14    
Medline Journal Info:
Nlm Unique ID:  9884881     Medline TA:  J Aquat Anim Health     Country:  United States    
Other Details:
Languages:  eng     Pagination:  258-73     Citation Subset:  IM    
Affiliation:
U.S. Geological Survey, National Fish Health Research Laboratory, Leetown, WV, USA.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed / analysis
Animal Nutritional Physiological Phenomena
Animals
Antibodies / metabolism*
Diet / veterinary
Fish Diseases / immunology*
Hemocyanin / chemistry,  immunology
Picrates / chemistry,  immunology
T-Lymphocytes / physiology*
Thiamine / administration & dosage,  pharmacology*
Thiamine Deficiency / immunology,  veterinary*
Trout / immunology*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Picrates; 59-43-8/Thiamine; 9013-72-3/Hemocyanin; A49OS0F91S/picric acid; FV4Y0JO2CX/keyhole-limpet hemocyanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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