Document Detail


Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients.
MedLine Citation:
PMID:  20496107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: According french legislation, sperm freezing/thawing procedures are used to prevent ART contaminations in couple with HIV-1 infected men. We determined sperm nuclear fragmentation rate before and after selection and freezing/thawing in HIV-1 14 patients.
METHODS: Two groups of patients were studied: 20 control patients with normal sperm (group 1) and without viral infection and 20 fertile treated HIV-1 patients (group 2). DNA fragmentation was evaluated using terminal uridine nick end labeling, before and after gradient selection, and after cryopreservation and thawing procedures.
RESULTS: DNA fragmentation rates in fresh semen were increased in HIV patients (6.38% vs 3.39%) (p < 0.05) compared with control patients. After sperm migration, fragmentation rates were significantly lower (p < 0.0001) in the two groups compared with fresh sperm rates. After freezing/thawing, values were similar to those of fresh semen with an increased rate (p < 0.01) for HIV-1 patients, with respectively 3.40% and 5.18% rates in control and infected patients. HIV-1-infected patients treated by antiretroviral therapy showed a significant increase in sperm DNA fragmentation in fresh sperm and also after freezing/thawing procedures, but these two fragmentation rates were not significantly different.
CONCLUSION: So, freezing/thawing procedures do not seem to impair sperm DNA and preserve probability of conception for couples with HIV-1 infected men.
Authors:
Christophe Frainais; François Vialard; Nathalie Rougier; Philippe Aegerther; Florence Damond; Jean-Philippe Ayel; Chadi Yazbeck; André Hazout; Jacqueline Selva
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Publication Detail:
Type:  Controlled Clinical Trial; Journal Article     Date:  2010-05-23
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  27     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-08-13     Completed Date:  2011-02-17     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  415-21     Citation Subset:  IM    
Affiliation:
Hôpital Bichat/Claude Bernard, Paris, France. christophefrainais@yahoo.fr
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MeSH Terms
Descriptor/Qualifier:
Adult
Cryopreservation / methods*
DNA / chemistry
DNA Fragmentation*
Fertility
Freezing
HIV Infections* / genetics
HIV-1*
Humans
Male
Semen / chemistry
Semen Preservation / methods*
Spermatozoa / chemistry*,  physiology
Chemical
Reg. No./Substance:
9007-49-2/DNA
Comments/Corrections

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