| Impact of seminal cytomegalovirus replication on HIV-1 dynamics between blood and semen. | |
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MedLine Citation:
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PMID: 22997072 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The genital tract of individuals infected with human immunodeficiency virus type 1 (HIV-1) is an anatomic compartment that supports local HIV-1 and cytomegalovirus (CMV) replication. This study investigated the association of seminal CMV replication with changes in HIV-1 clonal expansion, evolution and phylogenetic compartmentalization between blood and semen. Fourteen paired blood and semen samples were analyzed from four untreated subjects. Clonal sequences (n = 607) were generated from extracted HIV-1 RNA (env C2-V3 region), and HIV-1 and CMV levels were measured in the seminal plasma by real-time PCR. Sequence alignments were evaluated for: (i) viral compartmentalization between semen and blood samples using Slatkin-Maddison and F(ST) methods, (ii) different nucleotide substitution rates in semen and blood, and (iii) association between proportions of clonal HIV-1 sequences in each compartment and seminal CMV levels. Half of the semen samples had detectable CMV DNA, with at least one CMV positive sample for each patient. Seminal CMV DNA levels correlated positively with seminal HIV-1 RNA levels (Spearman P = 0.05). A trend towards an association between compartmentalization of HIV-1 sequences sampled from blood and semen and presence of seminal CMV was observed (Cochran Q test P = 0.12). Evolutionary rates between semen and blood HIV-1 populations did not differ significantly, and there was no significant association between seminal CMV DNA levels and the frequency of non-unique clonal HIV-1 sequences in the semen. In conclusion, the effects of CMV replication on HIV-1 viral and immunologic dynamics within the male genital tract are not significant enough to perturb evolution or disrupt compartmentalization in the genital tract. |
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Authors:
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Sara Gianella; Sanjay R Mehta; Matthew C Strain; Jason A Young; Milenka V Vargas; Susan J Little; Douglas D Richman; Sergei L Kosakovsky Pond; Davey M Smith |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of medical virology Volume: 84 ISSN: 1096-9071 ISO Abbreviation: J. Med. Virol. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-09-21 Completed Date: 2013-01-28 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: United States |
Other Details:
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Languages: eng Pagination: 1703-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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University of California San Diego, La Jolla, California 92093-0679, USA. gianella@ucsd.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
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Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood / virology* Cluster Analysis Coinfection / virology* Cytomegalovirus / isolation & purification* Cytomegalovirus Infections / complications, virology* Genotype HIV Infections / complications, virology* HIV-1 / classification, genetics, isolation & purification* Humans Male Molecular Sequence Data Phylogeny RNA, Viral / genetics Real-Time Polymerase Chain Reaction Semen / virology* Sequence Analysis, DNA Viral Load |
| Grant Support | |
ID/Acronym/Agency:
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AI043638/AI/NIAID NIH HHS; AI047745/AI/NIAID NIH HHS; AI077304/AI/NIAID NIH HHS; AI080353/AI/NIAID NIH HHS; AI36214/AI/NIAID NIH HHS; AI69432/AI/NIAID NIH HHS; AI74621/AI/NIAID NIH HHS; GM093939/GM/NIGMS NIH HHS; MH083552/MH/NIMH NIH HHS; MH62512/MH/NIMH NIH HHS; R01 MH083552/MH/NIMH NIH HHS; U01 AI043638/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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