Document Detail


Impact of seminal cytomegalovirus replication on HIV-1 dynamics between blood and semen.
MedLine Citation:
PMID:  22997072     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The genital tract of individuals infected with human immunodeficiency virus type 1 (HIV-1) is an anatomic compartment that supports local HIV-1 and cytomegalovirus (CMV) replication. This study investigated the association of seminal CMV replication with changes in HIV-1 clonal expansion, evolution and phylogenetic compartmentalization between blood and semen. Fourteen paired blood and semen samples were analyzed from four untreated subjects. Clonal sequences (n = 607) were generated from extracted HIV-1 RNA (env C2-V3 region), and HIV-1 and CMV levels were measured in the seminal plasma by real-time PCR. Sequence alignments were evaluated for: (i) viral compartmentalization between semen and blood samples using Slatkin-Maddison and F(ST) methods, (ii) different nucleotide substitution rates in semen and blood, and (iii) association between proportions of clonal HIV-1 sequences in each compartment and seminal CMV levels. Half of the semen samples had detectable CMV DNA, with at least one CMV positive sample for each patient. Seminal CMV DNA levels correlated positively with seminal HIV-1 RNA levels (Spearman P = 0.05). A trend towards an association between compartmentalization of HIV-1 sequences sampled from blood and semen and presence of seminal CMV was observed (Cochran Q test P = 0.12). Evolutionary rates between semen and blood HIV-1 populations did not differ significantly, and there was no significant association between seminal CMV DNA levels and the frequency of non-unique clonal HIV-1 sequences in the semen. In conclusion, the effects of CMV replication on HIV-1 viral and immunologic dynamics within the male genital tract are not significant enough to perturb evolution or disrupt compartmentalization in the genital tract.
Authors:
Sara Gianella; Sanjay R Mehta; Matthew C Strain; Jason A Young; Milenka V Vargas; Susan J Little; Douglas D Richman; Sergei L Kosakovsky Pond; Davey M Smith
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical virology     Volume:  84     ISSN:  1096-9071     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-09-21     Completed Date:  2013-01-28     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1703-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Data Bank Information
Bank Name/Acc. No.:
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MeSH Terms
Descriptor/Qualifier:
Adult
Blood / virology*
Cluster Analysis
Coinfection / virology*
Cytomegalovirus / isolation & purification*
Cytomegalovirus Infections / complications,  virology*
Genotype
HIV Infections / complications,  virology*
HIV-1 / classification,  genetics,  isolation & purification*
Humans
Male
Molecular Sequence Data
Phylogeny
RNA, Viral / genetics
Real-Time Polymerase Chain Reaction
Semen / virology*
Sequence Analysis, DNA
Viral Load
Grant Support
ID/Acronym/Agency:
AI043638/AI/NIAID NIH HHS; AI047745/AI/NIAID NIH HHS; AI077304/AI/NIAID NIH HHS; AI080353/AI/NIAID NIH HHS; AI36214/AI/NIAID NIH HHS; AI69432/AI/NIAID NIH HHS; AI74621/AI/NIAID NIH HHS; GM093939/GM/NIGMS NIH HHS; K23 AI093163/AI/NIAID NIH HHS; MH083552/MH/NIMH NIH HHS; MH62512/MH/NIMH NIH HHS; P01 AI074621/AI/NIAID NIH HHS; P30 AI036214/AI/NIAID NIH HHS; R01 MH083552/MH/NIMH NIH HHS; R56 AI047745/AI/NIAID NIH HHS; U01 AI043638/AI/NIAID NIH HHS; U01 AI069432/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Viral
Comments/Corrections

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