Document Detail

Impact of mitochondrial DNA on hypoxic radiation sensitivity in human fibroblast cells and osteosarcoma cell lines.
MedLine Citation:
PMID:  18497963     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to evaluate the impact of mitochondrial DNA (mtDNA) on radiation sensitivity under hypoxic conditions. The cell lines used were rho+ and rho0, which carry wild-type mtDNA and no mtDNA, respectively. The rho0 cells do not utilize oxygen because they lack the capacity to carry out oxidative phosphorylation. To confirm the role played by mtDNA in different cell lines, two types of cell line were used: human fibroblast and osteosarcoma cells. Radiosensitivity was evaluated by the colony formation assay, micronucleus (MN) formation assay and comet assay. Hypoxia lowered radiosensitivity in all three experiments for all four cell lines. Between rho+ and rho0 cells, no difference was found in the results from the colony formation assay and comet assay. However, higher MN formation was found in rho+ cells than in rho0 cells, not only under room air conditions in both the fibroblast and osteosarcoma cell lines, but also under hypoxic conditions. Therefore, although hypoxia lowers the radiosensitivity in general, the impact of mtDNA persists under hypoxic conditions.
Hideya Yamazaki; Ken Yoshida; Yasuo Yoshioka; Fumiaki Isohashi; Shuji Ozeki; Masahiko Koizumi; Makoto Yoneda; Takehiro Inoue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  19     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-23     Completed Date:  2008-08-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1545-9     Citation Subset:  IM    
Department of Radiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
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MeSH Terms
Bone Neoplasms / metabolism,  radiotherapy*
Cesium Radioisotopes
Colony-Forming Units Assay
Comet Assay
DNA, Mitochondrial / physiology*
Fibroblasts / metabolism,  radiation effects*
Gamma Rays
Mitochondria / metabolism*,  radiation effects
Osteosarcoma / metabolism,  radiotherapy*
Radiation Tolerance
Tumor Cells, Cultured
rho GTP-Binding Proteins / physiology
Reg. No./Substance:
0/Cesium Radioisotopes; 0/DNA, Mitochondrial; EC GTP-Binding Proteins

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