| Impact of microcystin containing diets on physiological performance of Nile tilapia (Oreochromis niloticus) concerning detoxification. | |
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MedLine Citation:
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PMID: 20949194 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Nile tilapia (Oreochromis niloticus) were fed by diets supplemented with cyanobacteria containing in part the cyanotoxin microcystin-LR (MC-LR) to determine the potential impacts on detoxification. Four different diets were prepared based on a commercial diet: (1) control, (2) MC-5% (containing 5% dried Microcystis sp. biomass with 4.92 μg MC-LR g(-1) diet), (3) MC-20% (containing 20% dried Microcystis sp. biomass with 19.54 μg MC-LR g(-1) diet), and (4) Arthr-20% (containing 20% dried Arthrospira sp. biomass without MC-LR). Blood and liver samples were taken after one, 7, and 28 days and protein has been determined in plasma and liver. In the liver, impacts on detoxification were measured by glutathione-S-transferase (GST) activities and gene expression of multi drug resistance protein (MDRP). Plasma protein did not change between all four diets at any sampling time whereas liver protein was significantly elevated already after one day in Arthr-20% and after 28 days in both, MC-20% and Arthr-20%. Biochemical measurements of GST activities revealed no significant impact at any sampling time. In order to characterize the potential effect of MC-LR on MDRP, RT-qPCR method was established. However, as for GST activities no significant changes in MDRP gene expression have been observed. Thus, in summary, oral exposure of MC-LR containing cyanobacteria to Nile tilapia via feed ingestion did not impact significantly detoxification in liver concerning GST activities and MDRP expression despite biochemical composition concerning liver protein was significantly elevated by the diets containing 20% cyanobacteria biomass, regardless whether they contained MC-LR or not. |
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Authors:
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Andrea Ziková; Achim Trubiroha; Claudia Wiegand; Sven Wuertz; Bernhard Rennert; Stephan Pflugmacher; Radovan Kopp; Jan Mareš; Petr Spurný; Werner Kloas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-15 |
Journal Detail:
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Title: Journal of environmental monitoring : JEM Volume: 12 ISSN: 1464-0333 ISO Abbreviation: J Environ Monit Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100968688 Medline TA: J Environ Monit Country: England |
Other Details:
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Languages: eng Pagination: 2276-81 Citation Subset: IM |
Affiliation:
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Department of Fisheries and Hydrobiology, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic. Andrea.Zikova@seznam.cz |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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