Document Detail


Impact of insemination technique, semen quality and oocyte cryopreservation on pronuclear morphology of zygotes derived from sibling oocytes.
MedLine Citation:
PMID:  19500447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pronuclear morphology seems to be an important predictive value of zygote development and integrity. In this study we want to evaluate the effect of insemination technique, male factor and oocyte cryopreservation on pronuclear morphology of zygotes derived from sibling oocytes in our Centre of Reproductive Medicine, Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy. Subjects (n = 190) were submitted to IVF cycles with non-frozen and frozen sibling oocytes. Morphological evaluations were assessed using zygote pronuclear morphology (pronuclei, nucleoli and axis) in four groups: Group 1: 144 zygotes from 85 conventional IVF cycles with non-frozen oocytes; Group 2: 164 zygotes from 85 intracytoplasmic sperm injection (ICSI) cycles with Group 1 patients' sibling frozen oocytes; Group 3: 221 zygotes from 123 ICSI cycles with non-frozen oocytes; Group 4: 197 zygotes from 123 ICSI cycles with Group 3 patients' sibling frozen oocytes. No differences between Group 1 and Group 2 were seen. Group 3 was statistically different from Group 4 in relation to the nucleolar morphology. Oocyte cryopreservation procedure modified the nucleolar morphology of zygotes only in the presence of poor semen quality.
Authors:
Alessia Nicoli; Francesco Capodanno; Barbara Valli; Roberta Di Girolamo; Maria Teresa Villani; Andrea Nucera; Riccardo Focarelli; Giovanni B La Sala
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Publication Detail:
Type:  Journal Article     Date:  2009-06-08
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  18     ISSN:  1469-8730     ISO Abbreviation:  Zygote     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-04     Completed Date:  2010-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  61-8     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy. nicoli.alessia@asmn.re.it
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Nucleus Shape*
Cells, Cultured
Cryopreservation
Female
Fertilization in Vitro / methods*
Humans
Male
Oocytes / physiology*
Spermatozoa / physiology*
Zygote / cytology*,  physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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