| Impact of hypoxia and hypercapnia on calcium oxalate toxicity in renal epithelial and interstitial cells. | |
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MedLine Citation:
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PMID: 16633808 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although there is an ongoing controversy about the primary site of calcium oxalate stone (CaOx) formation, there is some evidence for extratubular crystallization. However, the mechanisms leading to such interstitial calcifications are not clear. Anatomical studies have demonstrated a close association between the renal vasculature and renal tubules. It has been hypothesized that disorders of the vasculature may contribute to renal stone formation. The exceptional papillary environment with low oxygen and high carbon dioxide is of interest in this context and its impact on CaOx toxicity to renal cells has to be evaluated. LLC-PK1, Madin-Darby canine kidney (MDCK), human umbilical vein endothelial (HUVEC) and fibroblast cell lines were exposed to hypoxia (3% O2) alone, hypercapnia combined with hypoxia (3% O2, 18% CO2) or standard culture conditions (20% O2) for 72 h. Cell survival rates were determined microscopically after 4 h of incubation with CaOx at final concentrations of 1, 2 and 4 mM. DAPI staining and western blot were used to evaluate the induction of apoptosis. We confirmed that CaOx leads to concentration-dependent effects on the viability of the cell lines. HUVECs were most vulnerable to CaOx among the four cell lines. Incubation under hypoxia alone had no impact on CaOx toxicity to any of the cell lines in terms of survival. However, under combined hypoxic and hypercapnic conditions, all cell lines displayed a significant reduction of cell survival compared to room air incubation. Again, this effect was most pronounced for HUVECs. The induction of apoptosis could not be demonstrated in any experimental setting. Combined hypoxia and hypercapnia clearly aggravate CaOx toxicity to renal cell lines. As we could not demonstrate the induction of apoptosis, this effect may be a result of toxic necrosis. Especially the CaOx effect on interstitial cell lines might be of interest in the chronic ischemic papillary environment. An increased toxicity may lead to recurrent stone formation, and vice versa, diseases of the vasculature, like arteriosclerosis, may further promote stone formation by induction of local ischemia. This issue has to be clarified by further studies. |
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Authors:
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Yanwei Cao; Sreedhar Sagi; Axel Häcker; Annette Steidler; Peter Alken; Thomas Knoll |
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Publication Detail:
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Type: Journal Article Date: 2006-04-22 |
Journal Detail:
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Title: Urological research Volume: 34 ISSN: 0300-5623 ISO Abbreviation: Urol. Res. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-25 Completed Date: 2006-11-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364311 Medline TA: Urol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 271-6 Citation Subset: IM |
Affiliation:
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Department of Urology, Mannheim University Hospital, Theodor-Kutzer-Ufer 1-3, 68135, Mannheim, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia / metabolism* Calcium Oxalate / metabolism*, toxicity Cell Line Dogs Epithelial Cells / metabolism Humans Hypercapnia / metabolism* Kidney / metabolism* Swine Urinary Calculi / metabolism |
| Chemical | |
Reg. No./Substance:
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25454-23-3/Calcium Oxalate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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