Document Detail


Impact of exposure to bacteria on metabolism in the penaeid shrimp Litopenaeus vannamei.
MedLine Citation:
PMID:  16946240     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We hypothesized that aggregation of bacteria and hemocytes at the gill, which occurs as part of the shrimp's antibacterial immune defenses, would impair normal respiratory function and thereby disrupt aerobic metabolism. Changes in oxygen uptake and lactate accumulation were determined in Litopenaeus vannamei, the Pacific white shrimp, following injection with either saline (control) or a strain of the gram-negative bacterium Vibrio campbellii that is pathogenic in crustaceans. The rate of oxygen uptake was determined during the first 4 h after injection and after 24 h. Injection of bacteria decreased oxygen uptake by 27% (from 11.0 to 8.2 micromol g-1 h-1) after 4 h, while saline-injected shrimp showed no change. Decreased oxygen uptake persisted 24 h after Vibrio injection. In well-aerated water, resting whole-animal lactic acid levels increased in shrimp injected with bacteria (mean=2.59 micromol lactate g-1+/-0.39 SEM, n=8) compared to saline-injected control shrimp, but this difference did not persist at 24 h. Exposure to hypercapnic hypoxia (PCO2=1.8 kPa, PO2=6.7 kPa) also resulted in significant whole-body lactic acid differences (mean=3.99 and 1.8 micromol g-1 tissue in Vibrio and saline-injected shrimp, respectively). Our results support the hypothesis that the crustacean immune response against invading bacteria impairs normal metabolic function, resulting in depression of oxygen uptake and slightly increased anaerobic metabolism.
Authors:
David A Scholnick; Karen G Burnett; Louis E Burnett
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Biological bulletin     Volume:  211     ISSN:  0006-3185     ISO Abbreviation:  Biol. Bull.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-09-01     Completed Date:  2007-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984727R     Medline TA:  Biol Bull     Country:  United States    
Other Details:
Languages:  eng     Pagination:  44-9     Citation Subset:  IM    
Affiliation:
Marine Science, Eckerd College, St. Petersburg, Florida 33711, USA. David.Scholnick@pacificu.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide / metabolism
Lactic Acid / metabolism
Oxygen / analysis,  metabolism
Penaeidae / metabolism*,  microbiology*
Time Factors
Vibrio / metabolism*
Water / chemistry
Chemical
Reg. No./Substance:
124-38-9/Carbon Dioxide; 50-21-5/Lactic Acid; 7732-18-5/Water; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of beta-catenin on dendritic morphology and simulated firing patterns in cultured hippocampa...
Next Document:  Immune defense reduces respiratory fitness in Callinectes sapidus, the Atlantic blue crab.