Document Detail


Impact of cryopreservation on extracellular matrix structures of heart valve leaflets.
MedLine Citation:
PMID:  16488695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Transplantation of cryopreserved allografts represents a well-established valve replacement option. Despite their clinical use for more than 40 years, the integrity of the extracellular matrix (ECM) of these valves after thawing has not been determined. The purpose of this study was to investigate and compare ECM structures of fresh and cryopreserved porcine heart valve leaflets with special emphasis on the condition of collagenous and elastic fibers. METHODS: Pulmonary valves were excised from unprocessed porcine hearts under sterile conditions. After treatment with antibiotics, the valves were incubated in a cryoprotective solution, cryopreserved stepwise, and stored at -196 degrees C for 1 week. Two groups of heart valves (fresh untreated and thawed cryopreserved [each, n = 8]) were analyzed using biochemical (collagen, elastin, desmosine), histologic (hematoxylin-eosin, Movat-pentachrome, resorcin-fuchsin), and immunohistochemical (antibodies against collagen I, III, IV, and elastin) methods. Near-infrared femtosecond multiphoton laser scanning microscopy and second harmonic generation were used for high-resolution three-dimensional imaging of ECM structures. RESULTS: Biochemical testing demonstrated similar amounts of collagen and desmosine, but a minor loss of elastin in the cryopreserved specimens. Conventional histology revealed almost comparable cell and ECM formations in fresh and cryopreserved valve leaflets. In contrast, laser-induced autofluorescence imaging showed substantial ultrastructural deterioration and disintegration of most collagenous structures. Second harmonic generation was not inducible. CONCLUSIONS: Conventional cryopreservation of heart valves is accompanied by serious alterations and destruction of leaflet ECM structures, specifically demonstrated by multiphoton imaging. Further in-depth studies to clarify the impact of alternative cryopreservation techniques proposed for clinical use, such as vitrification, are crucial.
Authors:
Katja Schenke-Layland; Navid Madershahian; Iris Riemann; Barry Starcher; Karl-Jürgen Halbhuber; Karsten König; Ulrich A Stock
Related Documents :
15072365 - Micromachined transmissive scanning confocal microscope.
9453675 - Aluminum acid alizarin violet: a general purpose nuclear fluorochrome.
20113105 - A bright and long-pulse illumination for ultrahigh-speed microscopy of living specimens.
25461455 - In vivo imaging of transplanted stem cells in the central nervous system.
19039295 - Neuroimaging of cns involvement in hiv.
21089775 - Shading correction for on-board cone-beam ct in radiation therapy using planning mdct i...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  81     ISSN:  1552-6259     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-09-08     Revised Date:  2007-05-16    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  918-26     Citation Subset:  AIM; IM    
Affiliation:
Department of Cardiothoracic and Vascular Surgery, Friedrich-Schiller-University, Jena, Germany. kschenkelayland@mednet.ucla.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Collagen / analysis
Cryopreservation / methods*
Extracellular Matrix / ultrastructure*
Heart Valves / transplantation*,  ultrastructure*
Microscopy, Confocal
Models, Animal
Photons
Pulmonary Valve / ultrastructure
Swine
Transplantation, Homologous
Chemical
Reg. No./Substance:
9007-34-5/Collagen
Comments/Corrections
Comment In:
Ann Thorac Surg. 2006 Mar;81(3):927   [PMID:  16488696 ]
Ann Thorac Surg. 2007 May;83(5):1921; author reply 1921-2   [PMID:  17462446 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of temporary visceral ischemia on spinal cord ischemic damage in the rabbit.
Next Document:  Pyruvate mitigates oxidative stress during reperfusion of cardioplegia-arrested myocardium.