Document Detail


Impact of brief exercise on peripheral blood NK cell gene and microRNA expression in young adults.
MedLine Citation:
PMID:  23288554     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Natural killers (NK) cells are unique innate immune cells that increase up to fivefold in the circulating blood with brief exercise and are known to play a key role in first-response defense against pathogens and cancer immunosurveillance. Whether exercise alters NK cell gene and microRNA (miRNA) expression is not known. Thirteen healthy men (20-29 yr old) performed ten 2-min bouts of cycle ergometer exercise at a constant work equivalent to an average of 77% of maximum O2 consumption interspersed with 1-min rest. Blood was drawn before and immediately after the exercise challenge. NK cells were isolated from peripheral blood mononuclear cells using a negative magnetic cell separation method. We used Affymetrix U133+2.0 arrays for gene expression and Agilent Human miRNA V2 Microarray for miRNAs. A stringent statistical approach (false discovery rate < 0.05) was used to determine that exercise significantly altered the expression of 986 genes and 23 miRNAs. Using in silico analysis, we found exercise-related gene pathways where there was a high likelihood of gene-miRNA interactions. These pathways were predominantly associated with cancer and cell communication, including p53 signaling pathway, melanoma, glioma, prostate cancer, adherens junction, and focal adhesion. These data support the hypothesis that exercise affects the gene and miRNA expression pattern in the population of NK cells in the circulation and suggest mechanisms through which physical activity could alter health through the innate immune system.
Authors:
Shlomit Radom-Aizik; Frank Zaldivar; Fadia Haddad; Dan M Cooper
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-03
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  114     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-04     Completed Date:  2014-01-13     Revised Date:  2014-11-09    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  628-36     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Cytokines / blood
Exercise / physiology*
Gene Expression / genetics*,  immunology
Humans
Immunity, Innate / genetics,  immunology
Killer Cells, Natural / immunology,  metabolism,  physiology*
Lactic Acid / blood
Leukocytes / immunology
Leukocytes, Mononuclear / immunology,  physiology
Male
MicroRNAs / genetics*,  immunology
Oxygen Consumption / genetics,  immunology
Young Adult
Grant Support
ID/Acronym/Agency:
P01 HD048721/HD/NICHD NIH HHS; P01HD-048721/HD/NICHD NIH HHS; UL1 TR000153/TR/NCATS NIH HHS; UL1TR000153/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/MicroRNAs; 33X04XA5AT/Lactic Acid
Comments/Corrections

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