| Impact of blood sampling technique on blood quality and animal welfare in haemophilic mice. | |
| | |
MedLine Citation:
|
PMID: 21382833 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Blood collection in mice can be a challenge, in particular for samples used for coagulation analysis as initiation of coagulation during the procedures can influence the results. Blood collection from the retrobulbar venous plexus is commonly used but the method remains controversial. Several alternatives exist but not all are applicable to mice with a compromised coagulation system because of subsequently excessive bleeding. We therefore wanted to explore whether blood collection by puncture of the submandibular vein could replace blood collected from the retrobulbar venous plexus during pharmacokinetic and pharmacodynamic studies in mice lacking coagulation factor VIII (FVIII). The plasma concentrations of recombinant activated factor VII were independent of the blood collection method in a pharmacokinetic study. The same applied to the thromboelastographic profile of mice with normal coagulation in a pharmacodynamic study. However, excessive haemorrhages were observed in all FVIII knockout mice after a single puncture of the submandibular vein and 60% of the mice were euthanized 2-4 h after the blood collection. In contrast, no or only slight haemorrhage was observed in animals subjected to blood collection from the retrobulbar venous plexus. No signs of distress determined by blood glucose level or clinical abnormalities of the eye were observed after puncture of the retrobulbar venous plexus. In conclusion, blood collected by puncture of the submandibular vein and retrobulbar venous plexus has a quality which allows it to be used in coagulation assays. However, because of excessive bleedings, puncture of the submandibular vein is not recommended in mice lacking FVIII. |
| | |
Authors:
|
Heidi Holmberg; Maria Kristina Kiersgaard; Lars Friis Mikkelsen; Mikael Tranholm |
Related Documents
:
|
15104643 - Estimating blood donor loss due to the variant cjd travel deferral. 15881823 - Availability, safety and quality of blood for transfusion in the americas. 1418053 - Effects of mergocriptine on cerebral circulation and metabolism in cats. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-7 |
Journal Detail:
|
Title: Laboratory animals Volume: - ISSN: 1758-1117 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-8 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0112725 Medline TA: Lab Anim Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Haemostasis Pharmacology. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: No secular trend over the last decade in sperm counts among Swedish men from the general population.
Next Document: Quality assurance for the query and distribution systems of the RCSB Protein Data Bank.