| Impact of the arylhydrocarbon receptor on eugenol- and isoeugenol-induced cell cycle arrest in human immortalized keratinocytes (HaCaT). | |
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MedLine Citation:
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PMID: 16972787 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fragrances such as eugenol (4-allyl-2-methoxyphenol) and isoeugenol (2-methoxy-4-propenylphenol), naturally found in reasonable quantities in the essential oils of different spices, are not only common causes of contact dermatitis but also known for their antiproliferative actions. Previously, we found a cell cycle arrest and an arylhydrocarbon receptor (AhR)-mediated activation of cytochromes in immortalized keratinocytes (HaCaT) induced by both compounds. In the present study we investigated whether the cell cycle arrest of eugenol and isoeugenol is mediated by the AhR in HaCaT cells. Analysis of the cell cycle status by fluorescence-activated cell sorting (FACS) revealed an arrest of cells (32-34%) in the G0/G1 phase induced by both compounds. This was found in synchronized HaCaT cells, natural HaCaT, and siRNA AhR transfected HaCaT. The induced G0/G1 arrests were reduced in the presence of the highly selective AhR antagonist 3'-methoxy-4'-nitroflavone (MNF). In summary, these results, together with our previous findings that both compounds induce translocation of the AhR into the nucleus, provide good evidence that the effects of eugenol and isoeugenol in skin and keratinocytes are mediated by the AhR. Furthermore, these data suggest that the known growth suppressive effects of these compounds in some skin cells may be mediated by AhR interactions. |
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Authors:
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Michaela Kalmes; Andrea Neumeyer; Paola Rio; Helmut Hanenberg; Ellen Fritsche; Brunhilde Blömeke |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biological chemistry Volume: 387 ISSN: 1431-6730 ISO Abbreviation: Biol. Chem. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-15 Completed Date: 2008-01-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9700112 Medline TA: Biol Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 1201-7 Citation Subset: IM |
Affiliation:
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Department for Ecotoxicology and Toxicology, University of Trier, D-54296 Trier, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Benzo(a)pyrene
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pharmacology Cell Cycle / drug effects* Cell Line Cytochrome P-450 CYP1A1 / drug effects, genetics Dimethyl Sulfoxide / pharmacology Eugenol / analogs & derivatives*, pharmacology* Humans Keratinocytes / drug effects*, metabolism RNA, Messenger / drug effects, genetics Receptors, Aryl Hydrocarbon / drug effects*, metabolism Reverse Transcriptase Polymerase Chain Reaction / methods Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptors, Aryl Hydrocarbon; 50-32-8/Benzo(a)pyrene; 67-68-5/Dimethyl Sulfoxide; 97-53-0/Eugenol; 97-54-1/isoeugenol; EC 1.14.14.1/Cytochrome P-450 CYP1A1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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