| Impact of saturated, polyunsaturated and monounsaturated fatty acid-rich micelles on lipoprotein synthesis and secretion in Caco-2 cells. | |
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MedLine Citation:
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PMID: 19898980 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Meal fatty acids have been shown to modulate the size and composition of triacylglycerol (TAG)-rich lipoproteins influencing the magnitude and duration of the postprandial plasma TAG response. As a result there is considerable interest in the origin of these meal fatty-acid induced differences in particle composition. Caco-2 cells were incubated over 4 days with fatty acid mixtures resembling the composition of saturated (SFA), monounsaturated (MUFA) and polyunsaturated fatty acid (PUFA)-rich meals fed in a previous postprandial study to determine their impact on lipoprotein synthesis and secretion. The MUFA- and PUFA-rich mixtures supported greater intracellular TAG, but not cholesterol accumulation compared with the SFA-rich mixture (P < 0.001). The MUFA-rich mixture promoted significantly greater TAG and cholesterol secretion than the other mixtures and significantly more apolipoprotein B-100 secretion than the PUFA-rich mixture (P < 0.05). Electron microscopy revealed the SFA-rich mixture had led to unfavourable effects on cellular morphology, compared with the unsaturated fatty acid-rich mixtures. Our findings suggest the MUFA-rich mixture, may support the formation of a greater number of TAG-rich lipoproteins, which is consistent with indirect observations from our human study. Our electron micrographs are suggestive that some endocytotic uptake of MUFA-rich taurocholate micelles may promote greater lipoprotein synthesis and secretion in Caco-2 cells. |
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Authors:
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Kim G Jackson; Paul A Bateman; Parveen Yaqoob; Christine M Williams |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-08 |
Journal Detail:
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Title: Lipids Volume: 44 ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-01-21 Completed Date: 2010-04-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: Germany |
Other Details:
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Languages: eng Pagination: 1081-9 Citation Subset: IM |
Affiliation:
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Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, School of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK. k.g.jackson@reading.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Caco-2 Cells Cells / cytology, drug effects Fatty Acids / pharmacology* Fatty Acids, Monounsaturated / pharmacology* Fatty Acids, Unsaturated / pharmacology* Humans Lipoproteins / biosynthesis*, secretion Micelles* |
| Grant Support | |
ID/Acronym/Agency:
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45/D15215//Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 0/Fatty Acids, Unsaturated; 0/Lipoproteins; 0/Micelles |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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