Document Detail


Impact of repeated topical-loaded manganese-enhanced MRI on the mouse visual system.
MedLine Citation:
PMID:  22700708     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Optic nerve degeneration in diseases such as glaucoma and multiple sclerosis evolves in months to years. The use of Mn(2+)-Enhanced Magnetic Resonance Imaging (MEMRI) in a time-course study may provide new insights into the disease progression. Previously, we demonstrated the feasibility of using a topical administration for Mn(2+) delivery to the visual system. This study is to evaluate the impact of biweekly or monthly repeated Mn(2+) topical administration and the pH levels of the Mn(2+) solutions for MEMRI on the mouse visual pathway.
METHODS: Using groups of mice, the MEMRI with an acidic or pH neutralized 1 M MnCl(2) solution was performed. To evaluate the feasibility of repeated MEMRIs, topical-loaded MEMRI was conducted biweekly seven times or monthly three times. The enhancement of MEMRI in the visual system was quantified. After repeated MEMRIs, the corneas were examined by optical coherence tomography. The retinal ganglion cells (RGCs) and optic nerves were examined by histology.
RESULTS: All mice exhibited consistent enhancements along the visual system following repeated MEMRIs. The acidic Mn(2+) solution induced a greater MEMRI enhancement as compared with a neutral pH Mn(2+) solution. Significant 20% RGC loss was found after three biweekly Mn(2+) inductions, but no RGC loss was found after three monthly Mn(2+) treatments. The corneal thickness was found increased after seven biweekly topical-loaded MEMRI.
CONCLUSIONS: Acidic Mn(2+) solutions enhanced the uptake of Mn(2+) observed on the MEMRI. Increasing the time intervals of repeated Mn(2+) topical administration reduced the adverse effects caused by MEMRI.
Authors:
Shu-Wei Sun; Tiffany Thiel; Hsiao-Fang Liang
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-12
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-13     Completed Date:  2012-09-21     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4699-709     Citation Subset:  IM    
Affiliation:
Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA. rsun@llu.edu
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Animals
Contrast Media / adverse effects,  chemistry,  diagnostic use*
Female
Image Enhancement / methods*
Longitudinal Studies
Magnetic Resonance Imaging / methods*
Manganese Compounds / adverse effects,  chemistry,  diagnostic use*
Mice
Mice, Inbred C57BL
Optic Nerve / metabolism,  pathology
Retinal Ganglion Cells / metabolism,  pathology
Time Factors
Tomography, Optical Coherence
Visual Pathways / metabolism,  pathology
Grant Support
ID/Acronym/Agency:
R01 NS062830/NS/NINDS NIH HHS; R01 NS062830/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Manganese Compounds
Comments/Corrections

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