| The Impact of Porphyromonas Gingivalis Lipids on Apoptosis of Primary Human Chondrocytes. | |
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MedLine Citation:
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PMID: 22260531 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract The role of oral bacterial infections including periodontal disease (PD) in the pathogenesis of rheumatoid arthritis (RA) has gained increasing interest. Among the major periodontal pathogens, Porphyromonas gingivalis (P. gingivalis) has been mostly associated with RA pathogenesis. The aim of the present study was to analyze the effect of P. gingivalis total lipid fraction and dihydroceramides, as potent virulence factors, on human primary chondrocytes. Primary chondrocyte cultures were incubated with P. gingivalis phosphoglycerol dihydroceramide (PG DHC) lipids, the total lipid fraction (TL) or phosphoethanolamine dihydroceramide (PE DHC). Cell morphology changes were determined by phase contrast light microscopy. Early and late apoptosis cell analysis was performed by Annexin-V, active caspases and 7AAD staining, and examined by flow cytometry, and cell necrosis was evaluated by LDH release. Procaspase-3 activation was determined by Western blot analysis. Microscopic analysis showed altered cell morphology and cell shrinkage following incubation with P. gingivalis TL and PG DHC lipids. Flow cytometry demonstrated an increase of Annexin-V positive and active caspases positive chondrocytes after incubation with TL and the PG DHC fraction but not after PE DHC (control lipid) treatment or in untreated control cells. Furthermore, Western blot analysis showed an early cleavage of procaspase-3 after one hour. Significant LDH release following incubation with P. gingivalis lipids was demonstrated. The present data demonstrate that P. gingivalis lipids promote apoptosis in primary human chondrocytes, and thereby may contribute to the joint damage seen in the pathogenesis of RA. |
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Authors:
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Eric Röhner; Paula Hoff; Georg Matziolis; Carsten Perka; Birgit Riep; Frank C Nichols; Andrej M Kielbassa; Jacqueline Detert; Gerd R Burmester; Frank Buttgereit; Janine Zahlten; Nicole Pischon |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-19 |
Journal Detail:
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Title: Connective tissue research Volume: - ISSN: 1607-8438 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Traumatology and Orthopaedics, Charité - Universitätsmedizin , Berlin Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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